CAJ | 학술논문

目的：探讨HIF－1及下游SDF－1α／CXCR4和VEGF／VEGFR通路在介导DMOG动员MSCs中的作用机制。方法将雄性SD大鼠，随机分为五组：生理盐水对照组、DMOG组、YC－1组、AMD3100组、SU5416组。分别采用CFU－F法与流式细胞术检测大鼠骨髓和外周血中MSCs的数量；ELISA法检测骨髓上清及外周血清中SDF－1α及VEGF蛋白浓度；Western blotting法检测骨髓细胞中HIF－1α、SDF－1α及VEGF蛋白表达水平。结果同NS组比较，DMOG组CFU－Fs 数量显著增加，且CD45－CD90＋细胞群比例增加（ P ＜0．05）；同DMOG组比较， YC－1组、AMD3100组、SU5416组CFU－Fs数量均显著减少，且CD45－CD90＋细胞群比例降低（ P ＜0．05）；同DMOG组比较， YC－1组HIF－1α浓度及蛋白表达显著降低（ P ＜0．05）， AMD3100组 SDF－1α浓度及蛋白表达显著降低（ P ＜0．05），SU5416组VEGF浓度及蛋白表达显著降低（P ＜0．05）。结论 DMOG可能通过上调HIF－1α，从而调控其下游SDF－1α／CXCR4通路与VEGF／VEGFR通路诱导MSCs 的动员。
목적：탐토HIF－1급하유SDF－1α／CXCR4화VEGF／VEGFR통로재개도DMOG동원MSCs중적작용궤제。방법장웅성SD대서，수궤분위오조：생리염수대조조、DMOG조、YC－1조、AMD3100조、SU5416조。분별채용CFU－F법여류식세포술검측대서골수화외주혈중MSCs적수량；ELISA법검측골수상청급외주혈청중SDF－1α급VEGF단백농도；Western blotting법검측골수세포중HIF－1α、SDF－1α급VEGF단백표체수평。결과동NS조비교，DMOG조CFU－Fs 수량현저증가，차CD45－CD90＋세포군비례증가（ P ＜0．05）；동DMOG조비교， YC－1조、AMD3100조、SU5416조CFU－Fs수량균현저감소，차CD45－CD90＋세포군비례강저（ P ＜0．05）；동DMOG조비교， YC－1조HIF－1α농도급단백표체현저강저（ P ＜0．05）， AMD3100조 SDF－1α농도급단백표체현저강저（ P ＜0．05），SU5416조VEGF농도급단백표체현저강저（P ＜0．05）。결론 DMOG가능통과상조HIF－1α，종이조공기하유SDF－1α／CXCR4통로여VEGF／VEGFR통로유도MSCs 적동원。
Objective To explore the role of HIF-1 and its downstream SDF-1α/CXCR4 and VEGF/VEGFR pathway in mediating MSC mobilization with DMOG .Methods Male SD rats were randomly divided into five groups:Normal saline control group , DMOG group, YC-1 group, AMD3100 group, SU5416 group.We used CFU-F assay and flow cytometry to determine the number of MSCs in rat bone marrow ( BM ) and peripheral blood ( PB ) in each group , respectively.The concentrations of SDF-1αand VEGF both in BM and PB serum in each group were detected by ELISA . Western blotting was used to test protein levels of HIF-1α, SDF-1αand VEGF in BM.Results Compared with NS group, the number of CFU-Fs as well as the percentage of CD 45 -CD90 +cells increased in DMOG group ( P <0.05);Compared with DMOG group, the number of CFU-Fs as well as the percentage of CD 45 -CD90 +cells decreased in YC-1 group, AMD3100 group and SU5416 group (P <0.05).Compared with DMOG group, the concentration and protein expression of HIF-1αdecreased significantly in YC-1 group ( P <0.05 ) , the concentration and protein expression of SDF-1αdecreased significantly in AMD 3100 group ( P <0.05 ) , the concentration and protein expression of VEGF decreased significantly in SU5416 group ( P <0.05 ).Conclusion DMOG can induce MSCs mobilization possibly via up-regulating the expression of HIF-1αand activating its downstream SDF-1α/CXCR4 and VEGF/VEGFR pathway .