CAJ | 학술논문

目的：建立快速测定艾附暖宫丸中芍药苷含量的液相色谱法。方法采用 Bonshell ASB C18表面多孔壳层色谱柱（4．6×150mm，2．7μm），按照中国药典2010年版一部中艾附暖宫丸含量测定方法，以乙腈－0．1％磷酸溶液（12∶88）为流动相，等度洗脱，流速1．0mL? min －1，柱温30℃，检测波长230nm。结果样品溶液色谱图中显示与对照品溶液色谱图中相同的芍药苷色谱峰，且分离度良好。芍药苷在0．5～100．0μg? mL －1范围内与峰面积呈良好的线性关系（r＝0．9985）；重复性试验的 RSD＝1．4％（n＝6）；平均回收率为96．4％，RSD＝0．85％（n ＝6）。结论该方法快速、简便、灵敏、专属性强，可以实现艾附暖宫丸中芍药苷含量的快速检测。
목적：건립쾌속측정애부난궁환중작약감함량적액상색보법。방법채용 Bonshell ASB C18표면다공각층색보주（4．6×150mm，2．7μm），안조중국약전2010년판일부중애부난궁환함량측정방법，이을정－0．1％린산용액（12∶88）위류동상，등도세탈，류속1．0mL? min －1，주온30℃，검측파장230nm。결과양품용액색보도중현시여대조품용액색보도중상동적작약감색보봉，차분리도량호。작약감재0．5～100．0μg? mL －1범위내여봉면적정량호적선성관계（r＝0．9985）；중복성시험적 RSD＝1．4％（n＝6）；평균회수솔위96．4％，RSD＝0．85％（n ＝6）。결론해방법쾌속、간편、령민、전속성강，가이실현애부난궁환중작약감함량적쾌속검측。
OBJECTIVE To establish a RP-HPLC method for the fast determination of paeoniflorin in Aifu Nu-angong pills.METHODS The paeoniflorin was analyzed on a Bonshell ASB C 18 superficially porous layer core-shell column (4.6 ×150mm,2.7μm) by an acetonitrile-0.1%phosphoric acid (12∶88) as the mobile phase according to the 2010 edition of Chinese Pharmacopoeia.With 30℃column temperature,the flow rate was 1.0mL? min-1 and the UV detection wavelength was set at 230 nm.RESULTS The chromatogram of the sample solution showed the peak of paeoniflorin which was similar to the reference solution with high resolution .The linear range for the paeoniflorin was between 0.5μg? mL-1 and 100.0μg? mL-1 ( r=0.9985 ) ,the RSD of repeatability test was 1.4%,the average recovery was 96.4%and the RSD was 0.85%.CONCLUSION The method is rapid,simple,sensitive and high specificity ,which can be used in the fast determination of paeoniflorin in Aifu Nuangong pills .