CAJ | 학술논문

目的：建立抗内异合剂的质量控制方法。方法采用薄层色谱法对抗内异合剂中的莪术、三棱进行定性鉴别，采用高效液相色谱法测定制剂中丹参素的含量。色谱柱为Waters SymmetryC18（4．6mm ×250mm，5μm），流动相为甲醇∶水∶冰醋酸＝20∶80∶0．5，流速为1．0mL? min －1，检测波长为281nm。结果薄层色谱鉴别方法专属性强。丹参素在0．322～1．932μg范围内线性关系良好，r＝0．9996。丹参素加样回收率为97．18％，RSD为0．94％（n＝6）。结论该方法可行，结果准确，重复性好，可有效控制抗内异合剂的质量。
목적：건립항내이합제적질량공제방법。방법채용박층색보법대항내이합제중적아술、삼릉진행정성감별，채용고효액상색보법측정제제중단삼소적함량。색보주위Waters SymmetryC18（4．6mm ×250mm，5μm），류동상위갑순∶수∶빙작산＝20∶80∶0．5，류속위1．0mL? min －1，검측파장위281nm。결과박층색보감별방법전속성강。단삼소재0．322～1．932μg범위내선성관계량호，r＝0．9996。단삼소가양회수솔위97．18％，RSD위0．94％（n＝6）。결론해방법가행，결과준학，중복성호，가유효공제항내이합제적질량。
OBJECTIVE To establish the quality standard of Anti endometriosis Mixture .METHODS Curcu-ma,Spargan were identified by thin-layer chromatography ( TLC).HPLC method was established for the determina-tion of danshensu performed on Symmetry C 18 column (4.6mm ×250mm,5μm) with methanol-water-acetic acid (20∶80∶0.5) as the mobile phase at the flow rate of 1.0mL? min -1 ,column temperature at 30℃,and detection wave-length was 281nm.RESULTS The TLC method has high specificity .Within the range of 0.322~1.932μg,dansh-ensu presented a fine linear relationship ,r=0.9996.The recovery of danshensu was 97.18% with RSD 0.94%( n=6 ) respectively.CONCLUSION The methods are reliable ,accurate ,reproducible ,and can be used for the quali-ty control of Anti endometriosis Mixtures .