海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
12期
75-77
,共3页
薯蓣皂苷%正交试验法%超声波%HPLC
藷蕷皂苷%正交試驗法%超聲波%HPLC
서여조감%정교시험법%초성파%HPLC
Dioscin%Orthogonal design%Ultrasonic%HPLC
目的：研究穿山龙超声波醇提最佳提取工艺。方法采用超声波醇提工艺提取穿山龙中的有效成分，用高效液相色谱（ HPLC）测定薯蓣皂苷含量，以薯蓣皂苷含量为考察指标，采用L9（34）正交试验法考察提取因素对有效物质含量的影响，考察因素为提取溶剂乙醇的浓度、提取温度、提取的时间，从中优选出穿山龙超声波醇提最佳提取工艺条件。结果最佳的提取工艺用80％的乙醇提取2次，每次提取时间为30min，提取温度为70℃。提取的薯蓣皂苷平均含量1．89mg? g－1，RDS为3．592％，结论该提取工艺稳定、可靠。
目的：研究穿山龍超聲波醇提最佳提取工藝。方法採用超聲波醇提工藝提取穿山龍中的有效成分，用高效液相色譜（ HPLC）測定藷蕷皂苷含量，以藷蕷皂苷含量為攷察指標，採用L9（34）正交試驗法攷察提取因素對有效物質含量的影響，攷察因素為提取溶劑乙醇的濃度、提取溫度、提取的時間，從中優選齣穿山龍超聲波醇提最佳提取工藝條件。結果最佳的提取工藝用80％的乙醇提取2次，每次提取時間為30min，提取溫度為70℃。提取的藷蕷皂苷平均含量1．89mg? g－1，RDS為3．592％，結論該提取工藝穩定、可靠。
목적：연구천산룡초성파순제최가제취공예。방법채용초성파순제공예제취천산룡중적유효성분，용고효액상색보（ HPLC）측정서여조감함량，이서여조감함량위고찰지표，채용L9（34）정교시험법고찰제취인소대유효물질함량적영향，고찰인소위제취용제을순적농도、제취온도、제취적시간，종중우선출천산룡초성파순제최가제취공예조건。결과최가적제취공예용80％적을순제취2차，매차제취시간위30min，제취온도위70℃。제취적서여조감평균함량1．89mg? g－1，RDS위3．592％，결론해제취공예은정、가고。
OBJECTIVE To study the optimum ethanol extraction process by ultrasonic of Chuan shan-long.METHODS By Ethanol extraction by ultrasonic ,determination content of dioscin by HPLC ,the content of di-oscin as the detection targets ,the influence of extraction factors ( extraction concentration of solvent ethanol ,tempera-ture of the extraction ,and the time of the extraction number ) on the active substance content was investigated by or-thogonal design L9(34),which would optimize the extraction process.RESULTS The optimum extracting process for the Chuan shanlong was adding 80%ethanol,extracting 2 times,0.5 hours each times with extracting temperature 70℃.And the average content of dioscin was 1.89 mg? g -1 , RSD was 3.592%.CONCLUSION The process is stable and reliable.
