CAJ | 학술논문

目的：优化芦笋皂苷提取工艺，比较芦笋食用部分和废弃部分的皂苷含量。方法采用超声提取法，以芦笋中皂苷的提取率为指标，采用单因素实验和正交实验考察乙醇浓度、料液比、提取时间和提取温度对超声法提取芦笋两部分中总皂苷的影响，并用紫外分光光度法测定皂苷在两部分中的含量。结果芦笋食用部分总皂苷最佳提取条件为提取温度为70℃，提取时间为2h，料液比为1∶15，乙醇浓度为10％，提取率为179．83mg? g－1；芦笋废弃部分总皂苷最佳提取条件为提取温度为40℃，提取时间为2．5h，料液比为1∶15，乙醇浓度为30％，提取率为117．81 mg? g－1。结论经SPSS软件进行独立样本T检验，得知芦笋食用与废弃部分粗提物中皂苷的含量差异较大，需待进一步进行研究。
목적：우화호순조감제취공예，비교호순식용부분화폐기부분적조감함량。방법채용초성제취법，이호순중조감적제취솔위지표，채용단인소실험화정교실험고찰을순농도、료액비、제취시간화제취온도대초성법제취호순량부분중총조감적영향，병용자외분광광도법측정조감재량부분중적함량。결과호순식용부분총조감최가제취조건위제취온도위70℃，제취시간위2h，료액비위1∶15，을순농도위10％，제취솔위179．83mg? g－1；호순폐기부분총조감최가제취조건위제취온도위40℃，제취시간위2．5h，료액비위1∶15，을순농도위30％，제취솔위117．81 mg? g－1。결론경SPSS연건진행독립양본T검험，득지호순식용여폐기부분조제물중조감적함량차이교대，수대진일보진행연구。
OBJECTIVE To optimize the extraction process of the steroid saponins in asparagus , and compare the content of steroid saponins in edible part and disused part .METHODS With ultrasonic extraction ,viewed ex-traction rate as index.Using single factor experiment and orthogonal experiment to investigate the effect of ethanol concentration ,solid-liquid ratio ,extraction time and temperature in ultrasonic extraction method .And determination of the content of saponins in the two part by using UV Spectrophotometry .RESULTS The optimum extraction condi-tions of saponins in edible part were:70℃of extraction temperature ,extraction 2 hours,1∶15 of the ratio of solid to liquid with 10%alcohol concentration and the extraction rate was 179.83 mg? g -1;the optimum extraction conditions of saponins in waste part were:40℃of extraction temperature is,extraction 2.5 hours,1∶15 of the ratio of solid to liq-uid with 30%alcohol concentration and the extraction rate was 117.81 mg? g -1 .CONCLUSION Through analy-zing by via software SPSS ,the difference of steroid saponins content between edible part and disused part is huge ,a further research is required .