CAJ | 학술논문

目的：建立一种快速灵敏检测帕珠沙星血药物浓度的液相色谱－串联质谱方法。方法色谱柱为 Agilent Eclipse XDB－C18（4．6mm ×150mm，5μm）；流动相为乙腈：水（0．005mol? L－1甲酸铵，0．1％甲酸）＝（30∶70）；流速：0．5mL? min －1；质谱条件为电喷雾电离源（ESI）；采用选择反应监测（SRM）对帕珠沙星（m／z 319．0→301．1）和环丙沙星（m／z 332．2→314．2）进行测定。样本处理采用乙腈沉淀蛋白法，吸取上清液1μL进样。结果帕珠沙星的线性范围为0．02～20μg? mL －1，提取回收率为82％～92％，日内和日间精密度均小于15％。结论本方法简便、灵敏，该方法经考察符合血浆样品的测定要求，可以应用于临床药物浓度的测定和药代动力学的研究。
목적：건립일충쾌속령민검측파주사성혈약물농도적액상색보－천련질보방법。방법색보주위 Agilent Eclipse XDB－C18（4．6mm ×150mm，5μm）；류동상위을정：수（0．005mol? L－1갑산안，0．1％갑산）＝（30∶70）；류속：0．5mL? min －1；질보조건위전분무전리원（ESI）；채용선택반응감측（SRM）대파주사성（m／z 319．0→301．1）화배병사성（m／z 332．2→314．2）진행측정。양본처리채용을정침정단백법，흡취상청액1μL진양。결과파주사성적선성범위위0．02～20μg? mL －1，제취회수솔위82％～92％，일내화일간정밀도균소우15％。결론본방법간편、령민，해방법경고찰부합혈장양품적측정요구，가이응용우림상약물농도적측정화약대동역학적연구。
OBJECTIVE To establish a specific and sensitive HPLC-MS/MS method for determining the con-centration of pazufloxacin in human plasma .METHODS Pazufloxacin was extracted with acetonitrile .The drugs from plasma were separated on a Agilent Eclipse XDB-C18 (4.6mm ×150mm,5μm) column by using mobile phase consisted of acetonitrile (0.1%formic acid)-0.005mol? L-1 ammonium formate (30:70 V/V);the flow rate was 0.5 mL? min-1;an electrospray ionization ion-trap sourse was used as the detector and operated in the positive ion mode.Selected reacted monitoring ( SRM) was used to dectct pazufloxacin ( m/z 319.0→301.1 ) and ciprofloxacin ( m/z 332.2→314.2 ) .After protein sediment by acetonitrile ,1μL supernatantwas injected .RESULTS The cali-bration curves were linear in the range of 0.02~20μg? mL-1 .The extraction recovery was 82%~92%.The intra-day and inter-day variations (RSD) were less than 15%.CONCLUSION The method is sensitive,accurate,simple and quick ,it can be used for clinical drug monitoring and pharmacokinetics studies of pazufloxacin .