海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
12期
238-241
,共4页
隐丹参酮%非小细胞肺癌%流式细胞术%E-cadherin%β整合素
隱丹參酮%非小細胞肺癌%流式細胞術%E-cadherin%β整閤素
은단삼동%비소세포폐암%류식세포술%E-cadherin%β정합소
cryptotanshinone%non-small cell lung cancer%flow cytometry assay%E-cadherin%βintegrin
目的：研究隐丹参酮对非小细胞肺癌A549细胞生长、迁移、侵袭、细胞周期分布的影响及机制。方法以不同浓度的隐丹参酮作用于体外培养的A549细胞，采用MTT法检测细胞增殖活性的改变；划痕实验和Transwell实验分析细胞迁移、侵袭能力的改变；流式细胞仪检测细胞周期及E－cadherin和β整合素的表达情况。结果 MTT检测结果显示，一定浓度的隐丹参酮可抑制A549细胞的增殖，且随着浓度的增加其抑制作用明显增强。划痕实验和Transwell实验显示，A549细胞经隐丹参酮作用后，其迁移和侵袭能力受到抑制。流式细胞术显示，A549细胞经隐丹参酮作用后，细胞周期阻滞于G1期，G1期细胞比例上升，S期细胞比例下降，且药物作用后细胞表面的E－cadherin表达上调、β整合素的表达下降。结论隐丹参酮可抑制非小细胞肺癌A549细胞的增殖、迁移、侵袭，其机制可能与E－cadherin的上调和β整合素下调及阻滞细胞周期有关。
目的：研究隱丹參酮對非小細胞肺癌A549細胞生長、遷移、侵襲、細胞週期分佈的影響及機製。方法以不同濃度的隱丹參酮作用于體外培養的A549細胞，採用MTT法檢測細胞增殖活性的改變；劃痕實驗和Transwell實驗分析細胞遷移、侵襲能力的改變；流式細胞儀檢測細胞週期及E－cadherin和β整閤素的錶達情況。結果 MTT檢測結果顯示，一定濃度的隱丹參酮可抑製A549細胞的增殖，且隨著濃度的增加其抑製作用明顯增彊。劃痕實驗和Transwell實驗顯示，A549細胞經隱丹參酮作用後，其遷移和侵襲能力受到抑製。流式細胞術顯示，A549細胞經隱丹參酮作用後，細胞週期阻滯于G1期，G1期細胞比例上升，S期細胞比例下降，且藥物作用後細胞錶麵的E－cadherin錶達上調、β整閤素的錶達下降。結論隱丹參酮可抑製非小細胞肺癌A549細胞的增殖、遷移、侵襲，其機製可能與E－cadherin的上調和β整閤素下調及阻滯細胞週期有關。
목적：연구은단삼동대비소세포폐암A549세포생장、천이、침습、세포주기분포적영향급궤제。방법이불동농도적은단삼동작용우체외배양적A549세포，채용MTT법검측세포증식활성적개변；화흔실험화Transwell실험분석세포천이、침습능력적개변；류식세포의검측세포주기급E－cadherin화β정합소적표체정황。결과 MTT검측결과현시，일정농도적은단삼동가억제A549세포적증식，차수착농도적증가기억제작용명현증강。화흔실험화Transwell실험현시，A549세포경은단삼동작용후，기천이화침습능력수도억제。류식세포술현시，A549세포경은단삼동작용후，세포주기조체우G1기，G1기세포비례상승，S기세포비례하강，차약물작용후세포표면적E－cadherin표체상조、β정합소적표체하강。결론은단삼동가억제비소세포폐암A549세포적증식、천이、침습，기궤제가능여E－cadherin적상조화β정합소하조급조체세포주기유관。
OBJECTIVE To assess the effect of cryptotanshinone on cell growth , migration , invasion , and the cell cycle of non-small cell lung cancer cells.METHODS A549 cells were treated with different concentrations of cryptotanshinone in vitro .Cell proliferation was analyzed by MTT assay .Cell migration and invasion ability were eval-uated using wound scratch assay and transwell experiments .Flow cytometry was used to detect the change of cell cy-cle and the expression of E-cadherin and βintegrin.RESULTS The data from the MTT assay showed that crypto-tanshinone inhibited the proliferation of A 549 cells in an concentration dependent manner .Transwell experiments and wound scratch assay showed that cryptotanshinone could inhibit the migration and invasion ability of A 549 cells.Data from flow cytometry assay indicated that cryptotanshinone arrested cell cycle in G 1 phase ,rised proportion of cells in G1 phase and decreased proportion of cells in S phase .Furthermore,cryptotanshinone increased expression of E-cad-herin on the surface of cells ,while decreased expression of βintegrin.CONCLUSION Cryptotanshinone can inhib-it cell proliferation,migration and invasion ability of A549 cell.The mechanism may be related to cryptotanshinone effect on upregulation of E-cadherin ,downregulation of βintegrin and arrest of cell cycle .
