CAJ | 학술논문

【目的】为了优化生物丁醇生产工艺，研究拜氏梭菌（Clostridium beijerinckii ）对有毒物质的耐受性。【方法】利用常温等离子诱变技术选育获得1株突变株C．beijerinckii GZ-9，并通过摇瓶发酵对比原始菌株C． beijerinckii NCIMB 8052和突变株 GZ-9对有毒物质的耐受性。【结果】NCIMB 8052在酚浓度为1．5 g／L的发酵液中已不能生长，而 GZ-9在酚浓度2．4 g／L的发酵培养基中生长良好；当可溶性总酚浓度为1．5 g／L时，实验总溶剂产量和丁醇产量分别达9．2 g／L和6．5 g／L。【结论】突变株 GZ-9对未脱毒甘蔗渣酸解糖液中毒素物质的耐受性远高于原始菌株 NCIMB 8052。
【목적】위료우화생물정순생산공예，연구배씨사균（Clostridium beijerinckii ）대유독물질적내수성。【방법】이용상온등리자유변기술선육획득1주돌변주C．beijerinckii GZ-9，병통과요병발효대비원시균주C． beijerinckii NCIMB 8052화돌변주 GZ-9대유독물질적내수성。【결과】NCIMB 8052재분농도위1．5 g／L적발효액중이불능생장，이 GZ-9재분농도2．4 g／L적발효배양기중생장량호；당가용성총분농도위1．5 g／L시，실험총용제산량화정순산량분별체9．2 g／L화6．5 g／L。【결론】돌변주 GZ-9대미탈독감자사산해당액중독소물질적내수성원고우원시균주 NCIMB 8052。
Objective]The tolerance to toxic substances of Clostridium beijerinckii was studied in order to optimize bio-butanol industrial production.[Methods]A mutant strain C. beijerinckii GZ-9 was obtained by ion beam implantation and screening.Its inhibitors tolerance and butanol production were compared with that of the original strain C.beijerinckii NCIMB 8052.[Results]The results showed the strain NCIMB 8052 could not grow in the fermentation broth with the phenol concentration of 1 .5 g/L whereas the mutant GZ-9 grew well in fermen-tation medium with 2.4 g/L phenol.When the total soluble phenolic concentration was 1.5 g/L,the experiment yields of the total solvents and butanol production reached to 9.2 g/L and 6·5 g/L.[Conclusion]The mutant strain GZ-9 had strong tolerance to the toxic substances in non-detoxified acid sugar cane bagasse,which was much higher than the original strain NCIMB 8052.