广西科学
廣西科學
엄서과학
GUANGXI SCIENCES
2014年
6期
624-628
,共5页
吴兆鹏%曾练强%黄曾慰%常国炜%梁达奉%李雨虹
吳兆鵬%曾練彊%黃曾慰%常國煒%樑達奉%李雨虹
오조붕%증련강%황증위%상국위%량체봉%리우홍
毕赤酵母%α-葡聚糖酶%甘油%发酵条件
畢赤酵母%α-葡聚糖酶%甘油%髮酵條件
필적효모%α-포취당매%감유%발효조건
Pichia pastoris%α-glucanase%glycer-ol%fermentation conditions
【目的】考察甘油对组成型毕赤酵母(Pichiapastoris )生长和α-葡聚糖酶表达量的影响。【方法】采用单因素实验法考察初始发酵培养基中甘油浓度、补料阶段甘油残留和通气量等对α-葡聚糖酶表达量的影响。【结果】发酵培养基中初始甘油浓度从50 g/L提高到150 g/L,菌体生长和α-葡聚糖酶表达量均未受到影响,继续提高到200 g/L时,α-葡聚糖酶表达量明显下降。补料过程甘油残留在0~5 g/L,菌体生长和α-葡聚糖酶表达量最佳,当甘油残留较多时,菌体生长和α-葡聚糖酶的表达量均受到影响。提高通气量有利于增加α-葡聚糖酶的表达量,发酵78 h为宜,在此条件下α-葡聚糖酶酶活力达1763 U。【结论】该工艺优化了以甘油作为碳源制备α-葡聚糖酶的发酵条件,提高了α-葡聚糖酶酶活力,为大规模生产α-葡聚糖酶奠定了基础。
【目的】攷察甘油對組成型畢赤酵母(Pichiapastoris )生長和α-葡聚糖酶錶達量的影響。【方法】採用單因素實驗法攷察初始髮酵培養基中甘油濃度、補料階段甘油殘留和通氣量等對α-葡聚糖酶錶達量的影響。【結果】髮酵培養基中初始甘油濃度從50 g/L提高到150 g/L,菌體生長和α-葡聚糖酶錶達量均未受到影響,繼續提高到200 g/L時,α-葡聚糖酶錶達量明顯下降。補料過程甘油殘留在0~5 g/L,菌體生長和α-葡聚糖酶錶達量最佳,噹甘油殘留較多時,菌體生長和α-葡聚糖酶的錶達量均受到影響。提高通氣量有利于增加α-葡聚糖酶的錶達量,髮酵78 h為宜,在此條件下α-葡聚糖酶酶活力達1763 U。【結論】該工藝優化瞭以甘油作為碳源製備α-葡聚糖酶的髮酵條件,提高瞭α-葡聚糖酶酶活力,為大規模生產α-葡聚糖酶奠定瞭基礎。
【목적】고찰감유대조성형필적효모(Pichiapastoris )생장화α-포취당매표체량적영향。【방법】채용단인소실험법고찰초시발효배양기중감유농도、보료계단감유잔류화통기량등대α-포취당매표체량적영향。【결과】발효배양기중초시감유농도종50 g/L제고도150 g/L,균체생장화α-포취당매표체량균미수도영향,계속제고도200 g/L시,α-포취당매표체량명현하강。보료과정감유잔류재0~5 g/L,균체생장화α-포취당매표체량최가,당감유잔류교다시,균체생장화α-포취당매적표체량균수도영향。제고통기량유리우증가α-포취당매적표체량,발효78 h위의,재차조건하α-포취당매매활력체1763 U。【결론】해공예우화료이감유작위탄원제비α-포취당매적발효조건,제고료α-포취당매매활력,위대규모생산α-포취당매전정료기출。
Objective]The aim of this paper is mainly to investigate the effects of glycerol on Pichia pastoris growth and constitutive expression ofα-glucanase.[Methods]The initial concen-tration of glycerol in fermentation medium,glycerin concentration in fed-batch,ventilation and other effects on constitutive expression ofα-glucanase were examined by means of single factor experiment.[Results]When the initial concentration of glycerol ranged from 50 g/L to 150 g/L,the growth andα-glucanase expression were not affected,while the growth andα-glucanase expression appeared to be markedly reduced when the glycerol increase to 200 g/L.Glycerol concentration between 0 to 5 g/L in fed-batch lead to the best growth andα-glucanase expres-sion.When glycerin concentration became higher,the growth of Pichia pastoris andα-glu-canase expression were restrained.Meanwhile,improving the ventilation was helpful to the ex-pression of α - glucanase, and suitable fermentation time was 78 h. Under this condition,α-glucanase activity reached about 1763 U.[Conclusion]The process optimized the fermentation conditions forα-glucanase prepara-tion with glycerol as carbon source,which great-ly improvedα-glucanase activity and laid a solid foundation forα-glucanase large-scale produc-tion.