CAJ | 학술논문

【目的】改进肠膜明串珠菌（Leuconostoc mesenteriodes 31208）发酵蔗糖制备右旋糖酐的工艺，实现灵活控制分子量及高效制备右旋糖酐的目标。【方法】采用单因素实验法研究蔗糖浓度对蔗糖转化的影响，确定蔗糖流加工艺的各个参数，以及右旋糖酐酶添加耦合蔗糖流加的工艺条件。【结果】通过调控右旋糖酐酶添加工艺，可灵活控制右旋糖酐的分子量，无需再经酸水解及乙醇分级沉淀就可以得到不同分子量的右旋糖酐，节省无水乙醇的用量；且确定的最佳蔗糖流加工艺参数：初始蔗糖浓度为130 g／L，流加速度为12 g·L－1·h－1，起始流加时间为16 h。与蔗糖单批发酵结果相比，该工艺可使蔗糖转化效率提高约75％，特定分子量（5000～7500 Da）右旋糖酐浓度可达108 g／L，相应提高2．3倍，收率稳定在32％左右。【结论】该方法具有发酵易调控、高效和成本低的优点，对大规模生产右旋糖酐具有重要的参考价值和应用价值。
【목적】개진장막명천주균（Leuconostoc mesenteriodes 31208）발효자당제비우선당항적공예，실현령활공제분자량급고효제비우선당항적목표。【방법】채용단인소실험법연구자당농도대자당전화적영향，학정자당류가공예적각개삼수，이급우선당항매첨가우합자당류가적공예조건。【결과】통과조공우선당항매첨가공예，가령활공제우선당항적분자량，무수재경산수해급을순분급침정취가이득도불동분자량적우선당항，절성무수을순적용량；차학정적최가자당류가공예삼수：초시자당농도위130 g／L，류가속도위12 g·L－1·h－1，기시류가시간위16 h。여자당단비발효결과상비，해공예가사자당전화효솔제고약75％，특정분자량（5000～7500 Da）우선당항농도가체108 g／L，상응제고2．3배，수솔은정재32％좌우。【결론】해방법구유발효역조공、고효화성본저적우점，대대규모생산우선당항구유중요적삼고개치화응용개치。
Objective]This paper aims to control dextran molecular flexibly and prepare dextran efficiently with Leuconostoc mesenteriodes 31208 by improving the preparation process.[Meth-ods]The effect of sucrose concentration on sucrose conversion,the parameters of sucrose feed-ing,as well as the process conditions coupled enzymatic adding and sucrose feeding were stud-ied by means of single factor experiment.[Results]The molecular weight of dextran was con-trolled flexibly by changing the enzymatic hydrolysis process,and the parameters of optimal su-crose feeding process were the initial sucrose concentration of 130 g/L with the flow accelera-tion of 12 g·L-1 ·h-1 at 16 h.Compared with the single batch fermentation,the conversion rate improved by 75% and specific-molecular dextran(5000~7500 Da)increased over time,and the yield of dextran was about 32%.[Conclu-sion]This method has the characteristics of sim-ple fermentation control,high efficiency and low cost,which was important to the preparation of dextran for industrial production.