CAJ | 학술논문

目的：通过检测正常人群、梅毒未治疗患者、梅毒治疗后非梅毒螺旋体抗原血清试验转阴患者和梅毒血清固定患者血清IL－10、IL－12水平，并对梅毒血清固定患者血清 IL－10和IL－12作相关性分析。以探讨细胞免疫与梅毒血清固定的关系，为有效治疗梅毒血清固定患者提供一定的理论依据。方法：采用双抗体夹心ELISA法分别检测正常人群组、梅毒未治疗组、梅毒治疗转阴组和梅毒血清固定组的IL－10和IL－12水平，将各组IL－10和IL－12水平行统计学分析。对梅毒血清固定组中IL－10与IL－12作相关性分析。结果：梅毒血清固定组患者IL－10水平显著高于正常对照组（ P＜0．01）、治疗后阴转组（P＜0．01）及梅毒未治疗组（ P＜0．05）；梅毒血清固定组患者IL－12水平与正常对照组、治疗后阴转组及梅毒未治疗组之间差异无显著性（P＞0．05）；梅毒血清固定组患者IL－10与IL－12水平之间无明显相关性（r＝0．417，P＞0．05）。结论：梅毒血清固定组患者机体内细胞免疫受到一定程度的抑制，发生了免疫不平衡，这种免疫抑制和不平衡可能造成了梅毒螺旋体能够逃脱机体的免疫监视而残存，成为引起血清固定的原因之一。
목적：통과검측정상인군、매독미치료환자、매독치료후비매독라선체항원혈청시험전음환자화매독혈청고정환자혈청IL－10、IL－12수평，병대매독혈청고정환자혈청 IL－10화IL－12작상관성분석。이탐토세포면역여매독혈청고정적관계，위유효치료매독혈청고정환자제공일정적이론의거。방법：채용쌍항체협심ELISA법분별검측정상인군조、매독미치료조、매독치료전음조화매독혈청고정조적IL－10화IL－12수평，장각조IL－10화IL－12수평행통계학분석。대매독혈청고정조중IL－10여IL－12작상관성분석。결과：매독혈청고정조환자IL－10수평현저고우정상대조조（ P＜0．01）、치료후음전조（P＜0．01）급매독미치료조（ P＜0．05）；매독혈청고정조환자IL－12수평여정상대조조、치료후음전조급매독미치료조지간차이무현저성（P＞0．05）；매독혈청고정조환자IL－10여IL－12수평지간무명현상관성（r＝0．417，P＞0．05）。결론：매독혈청고정조환자궤체내세포면역수도일정정도적억제，발생료면역불평형，저충면역억제화불평형가능조성료매독라선체능구도탈궤체적면역감시이잔존，성위인기혈청고정적원인지일。
Objective:To investigate the relationship between cellular immunity and sero-resistance through determination of the IL-12 and IL-10 levels in healthy subjects,untreated syphilitic patients and those with either negative sera or serofast after therapy for theoretical evidence to management of the pa-tients with sero-resistance.Methods:Double-antibody sandwiched ELISA was used to detect the levels of IL-10 and IL-12 in healthy controls,patients with untreated syphilis and those with either negative sera or serofast after therapy .The IL-10 and IL-12 levels were statistically summarized in each group and correlatively analyzed in patients in their serofast state .Results:The serum level of IL-10 in syphilis patients with sero-resistance was significantly higher than that of the healthy controls and patients with negative sera after therapy as well as untreated syphilis (P<0.01 or P<0.05).No significant difference was observed regarding IL-12 levels in patients in serofast state,healthy controls,and patients with negative sera after therapy and untreated syphilitic pa-tients (P >0.05).There was no significant correlation in patients in serofast state concerning levels of IL-10 and IL-12(r =0.417,P >0.05). Conclusion:Cellular immunity imbalance and immune suppression can be present in syphilis patients with sero-resistance.This may be attributable to sur-vival of Treponema pallidum through escape from immune surveillance ,thus leading to sero-fast state for patients with syphilis after therapy .