皖南医学院学报
皖南醫學院學報
환남의학원학보
ACTA ACADEMIAE MEDICINAE WANNAN
2015年
1期
12-16
,共5页
双组分信号转导系统%SrrBA%表皮葡萄球菌%生物膜
雙組分信號轉導繫統%SrrBA%錶皮葡萄毬菌%生物膜
쌍조분신호전도계통%SrrBA%표피포도구균%생물막
two-component signal transduction system%SrrBA%staphylococcus epidermidis%biofilm
目的：探讨双组分信号转导系统SrrBA在表皮葡萄球菌中的调控作用。方法：利用同源重组技术构建表皮葡萄球菌srrBA基因敲除突变株，通过检测OD600值绘制其有氧生长曲线，并观察其厌氧生长状态，微量板半定量方法检测其生物膜形成能力。结果：经PCR扩增和测序验证获得了表皮葡萄球菌srrBA基因敲除突变株（ SE 1457－ΔsrrBA）。突变株的生长不论是在有氧还是在厌氧条件下均明显滞后于野生株。此外，突变株形成生物膜的能力较之野生株也显著下降。结论：双组分系统SrrBA可调控表皮葡萄球菌的生长和生物膜形成。
目的：探討雙組分信號轉導繫統SrrBA在錶皮葡萄毬菌中的調控作用。方法：利用同源重組技術構建錶皮葡萄毬菌srrBA基因敲除突變株，通過檢測OD600值繪製其有氧生長麯線，併觀察其厭氧生長狀態，微量闆半定量方法檢測其生物膜形成能力。結果：經PCR擴增和測序驗證穫得瞭錶皮葡萄毬菌srrBA基因敲除突變株（ SE 1457－ΔsrrBA）。突變株的生長不論是在有氧還是在厭氧條件下均明顯滯後于野生株。此外，突變株形成生物膜的能力較之野生株也顯著下降。結論：雙組分繫統SrrBA可調控錶皮葡萄毬菌的生長和生物膜形成。
목적：탐토쌍조분신호전도계통SrrBA재표피포도구균중적조공작용。방법：이용동원중조기술구건표피포도구균srrBA기인고제돌변주，통과검측OD600치회제기유양생장곡선，병관찰기염양생장상태，미량판반정량방법검측기생물막형성능력。결과：경PCR확증화측서험증획득료표피포도구균srrBA기인고제돌변주（ SE 1457－ΔsrrBA）。돌변주적생장불론시재유양환시재염양조건하균명현체후우야생주。차외，돌변주형성생물막적능력교지야생주야현저하강。결론：쌍조분계통SrrBA가조공표피포도구균적생장화생물막형성。
Objective:To explore the regulatory role of the two-component signal transduction system SrrBA in Staphylococcus epidermidis( S.epidermidis) . Methods:Homologous recombination technique was used to generate a srrBA knockout mutant in S.epidermidis 1457 via temperature-sensitive shuttle vec-tor pMAD.Bacterial growth curve was determined by measuring the value of OD600 under aerobic condition,with anaerobic growth being observed.Effect of srrBA mutant on the biofilm formation was measured by a semi-quantitative microtiter plate assay .Results:The knockout mutant was successfully acquired and verified by PCR amplification and sequencing.The mutant exhibited drastically retarded growth compared to the parent strain under either aerobic or anaerobic conditions.Moreover,biofilm formation was significantly reduced in the srrBA mutant.Conclusion:The SrrBA two-component system could regu-late bacterial growth and biofilm formation in S.epidermidis.
