海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2015年
3期
326-328
,共3页
吴海乔%田甜%胡君程%林蓁
吳海喬%田甜%鬍君程%林蓁
오해교%전첨%호군정%림진
热化%A549细胞%c-Jun N-末端激酶磷酸化%热休克蛋白70
熱化%A549細胞%c-Jun N-末耑激酶燐痠化%熱休剋蛋白70
열화%A549세포%c-Jun N-말단격매린산화%열휴극단백70
Thermotherapy%A549 cell%c-Jun N-terminal kinase phosphorylation%Heat shock protein 70
目的：观察热化联合对肺癌患者A549细胞生长的影响及机制探讨。方法对A549细胞分别进行单独热疗、单独化疗，热化联合干预及热化联合并SP600125干预，同时选取未做任何处理的A549细胞作为对照组。观察各组细胞增殖率、细胞侵袭力的变化。同时采用蛋白免疫印记法(Western Bolt）检测JNK磷酸化以及热休克蛋白70(HSP70)的表达。结果热化联合组的A549细胞增值率明显低于单独热疗、单独化疗和热化联合并SP600125组(P<0.05)。热化联合组JNK磷酸化表达明显高于对照组及单独化疗组(P<0.05)，热化联合组HSP70表达明显低于单独热疗组(P<0.05)。热化联合干预下，p-JNK表达水平出现上升，与对照组、单独热疗组和单独化疗组相比，差异均具有统计学意义(P<0.05)；热化联合并SP600125组的p-JNK的表达水平较热化联合组显著下降(P<0.05)。结论热化联合抑制A549细胞增殖的效果优于单独热疗或单独化疗，作用机制可能与激活JNK信号通路或抑制HSP70表达有关。
目的：觀察熱化聯閤對肺癌患者A549細胞生長的影響及機製探討。方法對A549細胞分彆進行單獨熱療、單獨化療，熱化聯閤榦預及熱化聯閤併SP600125榦預，同時選取未做任何處理的A549細胞作為對照組。觀察各組細胞增殖率、細胞侵襲力的變化。同時採用蛋白免疫印記法(Western Bolt）檢測JNK燐痠化以及熱休剋蛋白70(HSP70)的錶達。結果熱化聯閤組的A549細胞增值率明顯低于單獨熱療、單獨化療和熱化聯閤併SP600125組(P<0.05)。熱化聯閤組JNK燐痠化錶達明顯高于對照組及單獨化療組(P<0.05)，熱化聯閤組HSP70錶達明顯低于單獨熱療組(P<0.05)。熱化聯閤榦預下，p-JNK錶達水平齣現上升，與對照組、單獨熱療組和單獨化療組相比，差異均具有統計學意義(P<0.05)；熱化聯閤併SP600125組的p-JNK的錶達水平較熱化聯閤組顯著下降(P<0.05)。結論熱化聯閤抑製A549細胞增殖的效果優于單獨熱療或單獨化療，作用機製可能與激活JNK信號通路或抑製HSP70錶達有關。
목적：관찰열화연합대폐암환자A549세포생장적영향급궤제탐토。방법대A549세포분별진행단독열료、단독화료，열화연합간예급열화연합병SP600125간예，동시선취미주임하처리적A549세포작위대조조。관찰각조세포증식솔、세포침습력적변화。동시채용단백면역인기법(Western Bolt）검측JNK린산화이급열휴극단백70(HSP70)적표체。결과열화연합조적A549세포증치솔명현저우단독열료、단독화료화열화연합병SP600125조(P<0.05)。열화연합조JNK린산화표체명현고우대조조급단독화료조(P<0.05)，열화연합조HSP70표체명현저우단독열료조(P<0.05)。열화연합간예하，p-JNK표체수평출현상승，여대조조、단독열료조화단독화료조상비，차이균구유통계학의의(P<0.05)；열화연합병SP600125조적p-JNK적표체수평교열화연합조현저하강(P<0.05)。결론열화연합억제A549세포증식적효과우우단독열료혹단독화료，작용궤제가능여격활JNK신호통로혹억제HSP70표체유관。
Objective To investigate the effect of thermalization on A549 cells growth in patients with lung cancer and its mechanism. Methods A549 cells were given thermotherapy alone (group A), chemotherapy alone (group B), and thermotherapy combined with chemotherapy (group C), thermotherapy combined with chemotherapy and SP600125 intervention (group D). Untreated A549 cells were selected as the control group. The changes of cell in-vasion, proliferation rate of the cells in each group were observed. Phosphorylated JNK and expression of heat shock protein 70 (HSP70) were detected by Western blot. Results A549 cell proliferation rate of group C was significantly lower than that of group A, group B and group D (P<0.05). The expression of group C was significantly higher than that of control group and group B (P<0.05), and the expression of HSP70 in group C was significantly lower than that in group A (P<0.05). In group C, the expression level of p-JNK increased, compared with the control group, group A and group B, with statistically significant difference (P<0.05). In group D, the expression level of p-JNK decreased significantly compared with group C (P<0.05). Conclusion Thermotherapy combined with chemotherapy had better effects in inhibiting proliferation of A549 cells than thermotherapy or chemotherapy alone. The mechanism may be re-lated to the activation of JNK signal pathway or inhibiting expression of HSP70.
