CAJ | 학술논문

以红豆杉枝叶部位总多糖为原料，采用超滤方法对原料进行预处理，并对工艺进行优化，最佳优化条件为：截留相对分子质量为5kDa，温度为25℃，压力为20psi（1psi=6894.76kPa），pH值为6.8。在此条件下，将浓度为10g/L的1L红豆杉多糖溶液超滤至125mL，再添加去离子水至1L，反复超滤4次，得到的红豆杉多糖的截留率和脱色率分别为87.2%和75.6%。进一步以红豆杉单组分多糖间歇色谱分离工艺参数为基础，采用自行设计制作的连续色谱设备，建立了连续离子交换色谱分离红豆杉酸性单组分多糖PST-1的分离工艺，处理量达到每批次100.08L，PST-1纯度在99.0%以上，收率达到50.0%。
이홍두삼지협부위총다당위원료，채용초려방법대원료진행예처리，병대공예진행우화，최가우화조건위：절류상대분자질량위5kDa，온도위25℃，압력위20psi（1psi=6894.76kPa），pH치위6.8。재차조건하，장농도위10g/L적1L홍두삼다당용액초려지125mL，재첨가거리자수지1L，반복초려4차，득도적홍두삼다당적절류솔화탈색솔분별위87.2%화75.6%。진일보이홍두삼단조분다당간헐색보분리공예삼수위기출，채용자행설계제작적련속색보설비，건립료련속리자교환색보분리홍두삼산성단조분다당PST-1적분리공예，처리량체도매비차100.08L，PST-1순도재99.0%이상，수솔체도50.0%。
The total polysaccharides fromTaxus mairei were pretreated by ultrafiltration to remove pigments and the operating condition was optimized as follows:molecular cut-off 5 kDa,temperature 25℃,operating pressure 20psi and pH6.8. Under the aforementioned condition,the 1L solution containing 10g/L total polysaccharides was ultrafiltrated to 125mL first,then deionized water was added into condensed solution to 1L total volume and was repeated 4 times,respectively. The results showed that the retention rate and decolourization ratio of polysaccharides ofTaxus mairei were 87.2% and 75.6%,respectively. Furthermore,the pretreated total polysaccharides ofTaxus mairei were isolated by cation-exchange chromatography using a self-designed continuous chromatography equipment,the processing capacity of which reached 100.08L/d. Based on the operating parameters received from batch chromatography process,99.0% purity single-component polysaccharide(PST-1) was obtained and the average yield of PST-1 reached 50.0%.