化工进展
化工進展
화공진전
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2015年
2期
500-507,518
,共9页
α-淀粉酶抑制剂%内生放线菌%活性%筛选
α-澱粉酶抑製劑%內生放線菌%活性%篩選
α-정분매억제제%내생방선균%활성%사선
α-amylase inhibitor%endophytic actinomycetes%activity%screening
α-淀粉酶抑制剂属于糖苷水解酶抑制剂中的一种，能有效地抑制肠道内唾液及胰淀粉酶的活性，阻碍食物中碳水化合物的水解和消化，减少糖分的摄取，降低血糖和血脂含量水平，使食后不产生高血糖症。本实验利用快速灵敏的比色法，对从几种药用植物体内分离得到的70株内生放线菌菌丝提取物和发酵液的α-淀粉酶抑制活性进行了检测，得出：具有产胞外α-淀粉酶抑制剂活性的菌株19株，抑制率达70%以上的有6株；产胞内α-淀粉酶抑制剂活性的菌株16株，其中抑制率达70%以上的有3株；既能产胞内α-淀粉酶抑制剂又能产胞外α-淀粉酶抑制剂的菌株7株。从菌株WS19发酵液中分离获得了α-淀粉酶抑制剂WSI19，对α-淀粉酶和α-麦芽糖酶均有显著抑制，且对麦芽糖酶的抑制性比同剂量的奥恬苹还强11.2倍。经小鼠糖耐量实验，证实 WSI19对餐后高血糖有明显改善。总体上看，药用植物内生放线菌具有很大的产α-淀粉酶抑制剂潜力，可进一步为开发糖尿病药物。
α-澱粉酶抑製劑屬于糖苷水解酶抑製劑中的一種，能有效地抑製腸道內唾液及胰澱粉酶的活性，阻礙食物中碳水化閤物的水解和消化，減少糖分的攝取，降低血糖和血脂含量水平，使食後不產生高血糖癥。本實驗利用快速靈敏的比色法，對從幾種藥用植物體內分離得到的70株內生放線菌菌絲提取物和髮酵液的α-澱粉酶抑製活性進行瞭檢測，得齣：具有產胞外α-澱粉酶抑製劑活性的菌株19株，抑製率達70%以上的有6株；產胞內α-澱粉酶抑製劑活性的菌株16株，其中抑製率達70%以上的有3株；既能產胞內α-澱粉酶抑製劑又能產胞外α-澱粉酶抑製劑的菌株7株。從菌株WS19髮酵液中分離穫得瞭α-澱粉酶抑製劑WSI19，對α-澱粉酶和α-麥芽糖酶均有顯著抑製，且對麥芽糖酶的抑製性比同劑量的奧恬蘋還彊11.2倍。經小鼠糖耐量實驗，證實 WSI19對餐後高血糖有明顯改善。總體上看，藥用植物內生放線菌具有很大的產α-澱粉酶抑製劑潛力，可進一步為開髮糖尿病藥物。
α-정분매억제제속우당감수해매억제제중적일충，능유효지억제장도내타액급이정분매적활성，조애식물중탄수화합물적수해화소화，감소당분적섭취，강저혈당화혈지함량수평，사식후불산생고혈당증。본실험이용쾌속령민적비색법，대종궤충약용식물체내분리득도적70주내생방선균균사제취물화발효액적α-정분매억제활성진행료검측，득출：구유산포외α-정분매억제제활성적균주19주，억제솔체70%이상적유6주；산포내α-정분매억제제활성적균주16주，기중억제솔체70%이상적유3주；기능산포내α-정분매억제제우능산포외α-정분매억제제적균주7주。종균주WS19발효액중분리획득료α-정분매억제제WSI19，대α-정분매화α-맥아당매균유현저억제，차대맥아당매적억제성비동제량적오념평환강11.2배。경소서당내량실험，증실 WSI19대찬후고혈당유명현개선。총체상간，약용식물내생방선균구유흔대적산α-정분매억제제잠력，가진일보위개발당뇨병약물。
α-amylase inhibitor is a kind of glycosidase inhibitors. It can inhibit the activity of ptyalase and amylopsin,prevent the hydrolysis and digestion of carbohydrates,reduce the intake of sugar,so that depress postprandial blood glucose level. In this paper,theα-amylase inhibitory activity of the mycelium extracts and broth of 70 endophytic actinomycetes associated with medicinal plants was detected with rapid and sensitive colorimetry. The results showed that,there are 19 strains having the ability to produce extracellularα-amylase inhibitor,6 of them having more than 70% inhibion rate; there are 16 strains having the ability to produce intracellularα-amylase inhibitor,3 of them having more than 70% inhibion rate; there are 7 strains having the ability to produce both extracellular and intracellularα-amylase inhibitor. Theα-amylase inhibitor WSI19 was isolated and purified from the strain WS19,it could efficaciously inhibitα-amylase andα-maltase,and the inhibition of maltase is stronger than the same dose of miglitol 11.2 times. The glucose tolerance test in mice confirmed that WSI19 could significantly depress blood glucose levels. In a word,endophytic actinomycetes from medical plants have a better potential to produceα-amylase inhibitor,and could be developed to a possible therapeutic agent for diabetes.
