CAJ | 학술논문

目的：探讨七氟醚对幼鼠获取空间学习记忆能力及海马pERK1/2表达的影响。方法出生后14 d SD幼鼠54只，随机分为对照组(C组)、七氟醚5%组(Sevo组)、假手术组(Sham组)各18只。C组不做任何处理，Sevo组幼鼠暴露于5%的七氟醚4 h，Sham组幼鼠吸入氧气4 h，1周、3周、6周后于上午8：00~11：00应用Morris水迷宫对幼鼠进行7d的行为学观察，记录逃避潜伏期，行为学结束后随即取出6只幼鼠用免疫组化法测定海马CA1区神经元pERK1/2的表达。结果与Sham组比较，Sevo组逃避潜伏期和探索时间明显延长(P<0.01)，Sevo组海马CA1区神经元pERK1/2的表达明显减少(P<0.01)；Sham组与C组逃避潜伏期时间差异无统计学意义，海马CA1区神经元pERK1/2的表达之间差异无统计学意义。结论七氟醚对幼鼠获取空间学习记忆能力的影响与抑制海马pERK1/2表达有关。
목적：탐토칠불미대유서획취공간학습기억능력급해마pERK1/2표체적영향。방법출생후14 d SD유서54지，수궤분위대조조(C조)、칠불미5%조(Sevo조)、가수술조(Sham조)각18지。C조불주임하처리，Sevo조유서폭로우5%적칠불미4 h，Sham조유서흡입양기4 h，1주、3주、6주후우상오8：00~11：00응용Morris수미궁대유서진행7d적행위학관찰，기록도피잠복기，행위학결속후수즉취출6지유서용면역조화법측정해마CA1구신경원pERK1/2적표체。결과여Sham조비교，Sevo조도피잠복기화탐색시간명현연장(P<0.01)，Sevo조해마CA1구신경원pERK1/2적표체명현감소(P<0.01)；Sham조여C조도피잠복기시간차이무통계학의의，해마CA1구신경원pERK1/2적표체지간차이무통계학의의。결론칠불미대유서획취공간학습기억능력적영향여억제해마pERK1/2표체유관。
Objective To investigate effects of sevoflurane on spatial learning and memory ability and the expression of pERK1/2 in hippocampal in young SD rats. Methods Fifty-four young SD rats were randomly divided into three groups:Control group (C group), Sevoflurane group (Sevo group) and Sham operation group (Sham group), each with 18 rats. The rats in Sevo group were exposed in 5%sevoflurane for 4 hours, and the rats in S group were ex-posed in oxygen for 4 hours. Rats in three groups were taken to carry out the ethology observation for 7 days with Morris water maze 24 hours in 8:00~11:00 after 1 week, 3 weeks and 6 weeks of sevoflurane exposing, and the expres-sion of pERK1/2 was determined by immunochemistry. Results Compared with Sham group, the escape latency was significantly longer in Sevo group, and the expression of pERK1/2 was significantly inhibited in Sevo group (P<0.01). There was no statistically significant difference between C group and Sham group in the escape latency and the expres-sion of pERK1/2. Conclusion Effect of sevoflurane on spatial learning and memory ability of young SD rats is relat-ed to inhibition of pERK1/2 expression.