现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
2期
167-169
,共3页
癌,鳞状细胞%舌肿瘤%肿瘤蛋白质类%细胞增殖%细胞凋亡%流式细胞术%线粒体%熊果酸
癌,鱗狀細胞%舌腫瘤%腫瘤蛋白質類%細胞增殖%細胞凋亡%流式細胞術%線粒體%熊果痠
암,린상세포%설종류%종류단백질류%세포증식%세포조망%류식세포술%선립체%웅과산
Carcinoma,squamous cell%Tongue neoplasms%Neoplasm proteins%Cell proliferation%Apopotosis%Flow cytometry%Mitochondria%Ursolic acid
目的：探讨熊果酸对舌鳞癌Tca8113细胞增殖、凋亡的作用及可能机制，为临床治疗提供理论依据。方法经不同浓度熊果酸（10、20、40μmol/L）处理体外培养的舌鳞癌Tca8113细胞24 h后，采用MTT法检测细胞增殖活性，荧光染色法观察细胞形态学改变，流式细胞仪检测细胞凋亡率和线粒体膜电位的变化，Real-time PCR法检测Bcl-2和Bax mRNA的表达，酶联免疫吸附试验测定caspase-3蛋白酶活性。结果与对照组比较，不同浓度的熊果酸均可明显抑制Tca8113细胞增殖，诱导细胞DNA损伤，促进细胞凋亡，降低Bcl-2/Bax的表达比例，增高细胞线粒体膜电位（△Ψm），增强caspase-3蛋白酶活性，且具有一定的剂量依赖性。结论熊果酸可以抑制Tca8113细胞增殖并促进细胞凋亡，其机制可能与调控细胞线粒体凋亡途径有关。
目的：探討熊果痠對舌鱗癌Tca8113細胞增殖、凋亡的作用及可能機製，為臨床治療提供理論依據。方法經不同濃度熊果痠（10、20、40μmol/L）處理體外培養的舌鱗癌Tca8113細胞24 h後，採用MTT法檢測細胞增殖活性，熒光染色法觀察細胞形態學改變，流式細胞儀檢測細胞凋亡率和線粒體膜電位的變化，Real-time PCR法檢測Bcl-2和Bax mRNA的錶達，酶聯免疫吸附試驗測定caspase-3蛋白酶活性。結果與對照組比較，不同濃度的熊果痠均可明顯抑製Tca8113細胞增殖，誘導細胞DNA損傷，促進細胞凋亡，降低Bcl-2/Bax的錶達比例，增高細胞線粒體膜電位（△Ψm），增彊caspase-3蛋白酶活性，且具有一定的劑量依賴性。結論熊果痠可以抑製Tca8113細胞增殖併促進細胞凋亡，其機製可能與調控細胞線粒體凋亡途徑有關。
목적：탐토웅과산대설린암Tca8113세포증식、조망적작용급가능궤제，위림상치료제공이론의거。방법경불동농도웅과산（10、20、40μmol/L）처리체외배양적설린암Tca8113세포24 h후，채용MTT법검측세포증식활성，형광염색법관찰세포형태학개변，류식세포의검측세포조망솔화선립체막전위적변화，Real-time PCR법검측Bcl-2화Bax mRNA적표체，매련면역흡부시험측정caspase-3단백매활성。결과여대조조비교，불동농도적웅과산균가명현억제Tca8113세포증식，유도세포DNA손상，촉진세포조망，강저Bcl-2/Bax적표체비례，증고세포선립체막전위（△Ψm），증강caspase-3단백매활성，차구유일정적제량의뢰성。결론웅과산가이억제Tca8113세포증식병촉진세포조망，기궤제가능여조공세포선립체조망도경유관。
Objective To investigate the effect and possible mechanism of ursolic acid on proliferation and apopotosis of human tongue carcinoma cell line Tca8113 to provide theoretical reference for its clinic treatment. Methods After Tca8113 cells were incubated with various-concentration ursolic acid (10,20,40μmol/L) for 24 hours,the cell proliferation was examined by MTT colormetric assay. The morphology changes of chromosome were observed by invert fluorescent microscope. The apoptosis rate and mitochondrial transmembrane potential were measured by flow cytometry. The Bcl-2 and Bax mRNA expression was de-termined by real-time PCR. The activation of casepase-3 was detected with the casepase-3 activity assay kit. Results In com-parison with the control group,the ursolic acid with different concentrations could obviously inhibit the Tca8113 cells apoptosis, induce the injury of cells DNA,accelerate the cells apoptosis,lower the expression rate of Bcl-2/Bax,raise the mitochondrial trans membrane potential(△Ψm),and strengthen the casepase-3 proteinase activity in a ursolic acid dose-dependent manner. Conclu-sion Ursolic acid may inhibit Tca8113 cell proliferation and accelerate the cells apoptosis,which might be correlated with adjust-ment and regulation of apoptosis in the path of cell mitochondrion.
