中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2015年
2期
4-7
,共4页
乳腺癌%增殖%膜联蛋白域
乳腺癌%增殖%膜聯蛋白域
유선암%증식%막련단백역
Breast cancer%Multiplication%AnnexinII
目的探讨膜联蛋白Ⅱ在乳腺癌中的表达以及对乳腺癌细胞侵袭的影响。方法分别采用免疫组化方法检测AnnexinⅡ蛋白在正常乳腺组织、导管原位癌以及侵袭性导管癌组织中的表达情况，并测定高侵袭性乳腺癌细胞株及低侵袭性乳腺癌细胞株中AnnexinⅡ mRNA(RT-PCR法)以及蛋白(蛋白印记技术)的表达情况。结果 AnnexinⅡ在正常乳腺组织中主要表达在间质内，在乳腺导管上不表达，在导管原位癌以及侵袭性导管癌组织中间质、血管、肿瘤组织上均有表达。 AnnexinⅡ在正常乳腺组织表达的IOD值为(6.5±1.0)×106，在导管原位癌中表达的IOD值为(27.5±3.0)×106，在侵袭性导管癌组织中表达的IOD值为(40.0±11.0)×106。三组间比较，差异有高度统计学意义(P<0.01)，导管原位癌、侵袭性导管癌组织中的表达量显著高于正常乳腺组织，而侵袭性导管癌中的表达量显著高于导管原位癌(均P<0.01)。 AnnexinⅡmRNA在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达灰度值(0.83±0.11)显著高于MCF-7株中的表达(0.07±0.01)，差异有高度统计学意义(P<0.01)；An-nexinⅡ蛋白在高侵袭性人乳腺癌细胞株MDA-MB-435s中的表达(1.47±0.08)显著高于MCF-7株中的表达(0.11±0.03)，差异有高度统计学意义(P<0.01)。结论 AnnexinⅡ在乳腺癌中高表达，并且瘤细胞侵袭性越强，其表达也越强。
目的探討膜聯蛋白Ⅱ在乳腺癌中的錶達以及對乳腺癌細胞侵襲的影響。方法分彆採用免疫組化方法檢測AnnexinⅡ蛋白在正常乳腺組織、導管原位癌以及侵襲性導管癌組織中的錶達情況，併測定高侵襲性乳腺癌細胞株及低侵襲性乳腺癌細胞株中AnnexinⅡ mRNA(RT-PCR法)以及蛋白(蛋白印記技術)的錶達情況。結果 AnnexinⅡ在正常乳腺組織中主要錶達在間質內，在乳腺導管上不錶達，在導管原位癌以及侵襲性導管癌組織中間質、血管、腫瘤組織上均有錶達。 AnnexinⅡ在正常乳腺組織錶達的IOD值為(6.5±1.0)×106，在導管原位癌中錶達的IOD值為(27.5±3.0)×106，在侵襲性導管癌組織中錶達的IOD值為(40.0±11.0)×106。三組間比較，差異有高度統計學意義(P<0.01)，導管原位癌、侵襲性導管癌組織中的錶達量顯著高于正常乳腺組織，而侵襲性導管癌中的錶達量顯著高于導管原位癌(均P<0.01)。 AnnexinⅡmRNA在高侵襲性人乳腺癌細胞株MDA-MB-435s中的錶達灰度值(0.83±0.11)顯著高于MCF-7株中的錶達(0.07±0.01)，差異有高度統計學意義(P<0.01)；An-nexinⅡ蛋白在高侵襲性人乳腺癌細胞株MDA-MB-435s中的錶達(1.47±0.08)顯著高于MCF-7株中的錶達(0.11±0.03)，差異有高度統計學意義(P<0.01)。結論 AnnexinⅡ在乳腺癌中高錶達，併且瘤細胞侵襲性越彊，其錶達也越彊。
목적탐토막련단백Ⅱ재유선암중적표체이급대유선암세포침습적영향。방법분별채용면역조화방법검측AnnexinⅡ단백재정상유선조직、도관원위암이급침습성도관암조직중적표체정황，병측정고침습성유선암세포주급저침습성유선암세포주중AnnexinⅡ mRNA(RT-PCR법)이급단백(단백인기기술)적표체정황。결과 AnnexinⅡ재정상유선조직중주요표체재간질내，재유선도관상불표체，재도관원위암이급침습성도관암조직중간질、혈관、종류조직상균유표체。 AnnexinⅡ재정상유선조직표체적IOD치위(6.5±1.0)×106，재도관원위암중표체적IOD치위(27.5±3.0)×106，재침습성도관암조직중표체적IOD치위(40.0±11.0)×106。삼조간비교，차이유고도통계학의의(P<0.01)，도관원위암、침습성도관암조직중적표체량현저고우정상유선조직，이침습성도관암중적표체량현저고우도관원위암(균P<0.01)。 AnnexinⅡmRNA재고침습성인유선암세포주MDA-MB-435s중적표체회도치(0.83±0.11)현저고우MCF-7주중적표체(0.07±0.01)，차이유고도통계학의의(P<0.01)；An-nexinⅡ단백재고침습성인유선암세포주MDA-MB-435s중적표체(1.47±0.08)현저고우MCF-7주중적표체(0.11±0.03)，차이유고도통계학의의(P<0.01)。결론 AnnexinⅡ재유선암중고표체，병차류세포침습성월강，기표체야월강。
Objective To discuss expression of Annexin II in breast cancer and the effects on the invasion of breast cancer cells. Methods Annexin II expression in normal breast tissue, ductal carcinoma in situ and invasive ductal carcinoma tissues were detected by immunohistochemical method, and Annexin II mRNA (RT-PCR) and protein (Western blot) expressions in high invasive breast cancer cell lines and low invasive breast cancer cell lines were detected. Results In normal breast tissue, Annexin II was mainly detected in troma, without expression on breast catheter. In ductal carcinoma in situ and invasive ductal carcinoma tissues, Annexin II was detected in troma, vessel and tumor tissue. IOD of Annexin II expression in normal breast tissue was (6.5±1.0)í106, in ductal carcinoma was (27.5±3.0)í106, and in invasive ductal carcinoma tissues was (40.0±11.0)í106. There was significant difference in three groups (P<0.01). IOD of AnnexinII expression in invasive ductal carcinoma tissues and ductal carcinoma was higher than that in normal breast tissue, and IOD of Annexin II expression in invasive ductal carcinoma tissues was higher than that in ductal carcinoma (all P<0.01). Gray value of AnnexinII mRNA expression in high invasive breast cancer cell lines MDA-MB-435s was (0.83±0.11), which was higher than that in low invasive breast cancer cell lines MCF-7 (0.07±0.01), and there was significant difference between two cell lines (P<0.01). IOD of AnnexinIIprotein expression in high invasive breast cancer cell lines MDA-MB-435s was (1.47±0.08), which was higher than that in low invasive breast cancer cell lines MCF-7 (0.11±0.03), and there was significant difference between two cell lines (P<0.01). Conclusion AnnexinII shows high expression in breast cancer, and the stronger aggressive of tumor cells is, the higher expression of it is.
