中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2015年
1期
8-11
,共4页
喉鳞状细胞癌%存活素%基质金属蛋白酶-2%免疫组织化学
喉鱗狀細胞癌%存活素%基質金屬蛋白酶-2%免疫組織化學
후린상세포암%존활소%기질금속단백매-2%면역조직화학
Laryngeal squamous cell carcinoma%Survivin%Matrix metalloproteinase-2%Immunohis-tochemistry
目的：探讨喉癌组织中存活素(Survivin)和基质金属蛋白酶-2(MMP-2)的表达及其相关性。方法采用免疫组织化学链酶卵白素-生物素复合体(SABC)法检测Survivin、MMP-2蛋白在92例喉鳞状细胞癌及相距10 mm的癌旁组织和25例喉正常黏膜中的表达。结果Survivin在喉癌组织中的阳性率(76.09%)明显高于癌旁(13.04%)及喉正常黏膜组织(8.00%)，差异有统计学意义(P<0.05)，与临床分期有关，与年龄、淋巴结转移、分化程度无关。MMP-2在喉癌组织中的阳性率(78.26%)明显高于癌旁(18.48%)及喉正常黏膜组织(12.00%)，差异有统计学意义(P<0.05)，与分化程度和临床分期有关，与年龄、淋巴结转移无关。两者呈正相关。结论 Survivin、MMP-2的异常表达可能对喉癌的发生、发展起重要作用，联合检测可能对喉癌的早期诊断、进一步治疗和判断预后有一定参考价值。
目的：探討喉癌組織中存活素(Survivin)和基質金屬蛋白酶-2(MMP-2)的錶達及其相關性。方法採用免疫組織化學鏈酶卵白素-生物素複閤體(SABC)法檢測Survivin、MMP-2蛋白在92例喉鱗狀細胞癌及相距10 mm的癌徬組織和25例喉正常黏膜中的錶達。結果Survivin在喉癌組織中的暘性率(76.09%)明顯高于癌徬(13.04%)及喉正常黏膜組織(8.00%)，差異有統計學意義(P<0.05)，與臨床分期有關，與年齡、淋巴結轉移、分化程度無關。MMP-2在喉癌組織中的暘性率(78.26%)明顯高于癌徬(18.48%)及喉正常黏膜組織(12.00%)，差異有統計學意義(P<0.05)，與分化程度和臨床分期有關，與年齡、淋巴結轉移無關。兩者呈正相關。結論 Survivin、MMP-2的異常錶達可能對喉癌的髮生、髮展起重要作用，聯閤檢測可能對喉癌的早期診斷、進一步治療和判斷預後有一定參攷價值。
목적：탐토후암조직중존활소(Survivin)화기질금속단백매-2(MMP-2)적표체급기상관성。방법채용면역조직화학련매란백소-생물소복합체(SABC)법검측Survivin、MMP-2단백재92례후린상세포암급상거10 mm적암방조직화25례후정상점막중적표체。결과Survivin재후암조직중적양성솔(76.09%)명현고우암방(13.04%)급후정상점막조직(8.00%)，차이유통계학의의(P<0.05)，여림상분기유관，여년령、림파결전이、분화정도무관。MMP-2재후암조직중적양성솔(78.26%)명현고우암방(18.48%)급후정상점막조직(12.00%)，차이유통계학의의(P<0.05)，여분화정도화림상분기유관，여년령、림파결전이무관。량자정정상관。결론 Survivin、MMP-2적이상표체가능대후암적발생、발전기중요작용，연합검측가능대후암적조기진단、진일보치료화판단예후유일정삼고개치。
Objective To investigate the expression and correlation of survivin and matrix metalloproteinase-2 (MMP-2) in laryngeal carcinoma. Methods Immunohistochemical strept avidin-biotin complex (SABC) was used to detect the ex-pression of Survivin,MMP-2 protein in 92 cases of laryngeal squamous cell carcinoma and 10 mm away from the tumor adjacent tissues and 25 cases of normal laryngeal mucosa. Results The positive rate of Survivin in laryngeal carcinoma (76.09%) was higher than that in para carcinoma (13.04%) and normal laryngeal mucosa (8%),the difference was sig-nificant (P<0.05),was related to clinical staging,and age,lymph node metastasis,unrelated to differentiation.The positive rate of MMP-2 in laryngeal carcinoma (78.26%) was higher than that in para carcinoma (18.48%) and normal laryngeal mucosa (12.00%),the difference was significant (P<0.05),was associated with clinical stages and differentiation level,but not with age,lymph node metastasis.Survivin was positively correlated with MMP-2. Conclusion The abnormal expres-sion of Survivin MMP-2 in the carcinogenesis of laryngeal squamous cell carcinoma may play an important role in the development of laryngeal carcinoma,the combined detection of possible early diagnosis,further treatment and prognosis judgment of a certain reference value.
