CAJ | 학술논문

为了探索植物生长调节剂对后熟过程中猕猴桃电阻抗图谱的影响，检测了膨大果和未处理的对照果的电阻抗图谱，观察了2类猕猴桃果肉组织细胞微观结构的变化，利用Hayden等效电路模型分析了后熟过程中猕猴桃果肉组织细胞外液电阻、细胞内液电阻和细胞膜阻抗的电学特性变化。后熟过程中，低频时膨大果阻值较大但变化较小，高频时2类猕猴桃阻值趋于一致；频率为12kHz时相位角最大；2类果的Cole-cole图均为一段圆弧，对照果的圆弧半径变化较大；膨大果细胞膜阻抗差异不显著，对照果从第7天开始细胞膜阻抗急剧减小；对照果细胞外液电阻低于膨大果，从第7天开始2类果均呈现减小趋势。电阻抗图谱法揭示了后熟过程中对照果与膨大果的电阻抗特性变化规律，为猕猴桃膨大果的检测识别提供了研究基础。
위료탐색식물생장조절제대후숙과정중미후도전조항도보적영향，검측료팽대과화미처리적대조과적전조항도보，관찰료2류미후도과육조직세포미관결구적변화，이용Hayden등효전로모형분석료후숙과정중미후도과육조직세포외액전조、세포내액전조화세포막조항적전학특성변화。후숙과정중，저빈시팽대과조치교대단변화교소，고빈시2류미후도조치추우일치；빈솔위12kHz시상위각최대；2류과적Cole-cole도균위일단원호，대조과적원호반경변화교대；팽대과세포막조항차이불현저，대조과종제7천개시세포막조항급극감소；대조과세포외액전조저우팽대과，종제7천개시2류과균정현감소추세。전조항도보법게시료후숙과정중대조과여팽대과적전조항특성변화규률，위미후도팽대과적검측식별제공료연구기출。
Plant growth regulators were used frequently to improve the yield of kiwifruits in recent years. But the use of plant growth regulators often caused low internal quality and deformity of fruits, and the expanded kiwifruits easily decay after softening. It is necessary to explore the effect of plant growth regulators on kiwifruit tissue during the ripening process. Up to now, the traditional chemical methods and the other modern techniques such as NIR, FTIR and NMR have been adopted to analyze the influence of plant growth regulators on fruit tissues. But these approaches are difficult to achieve fast and cheap measurement. Electrical impedance spectroscopy (EIS) is a fast developing method in analyzing materials' characterization. When currents with different frequencies flow through the biological tissue, cytomembrane, the extracellular and intracellular fluid would show different electrical properties. Therefore, EIS has the potential to be used for detecting the change of kiwifruit cell microstructure during the ripening process. To develop a quick, convenient and economic method for exploring the effect of plant growth regulators on kiwifruit fruit, EIS technique was used to analyze the change of biological tissue at cellular level during the ripening process of the expanded and control kiwifruits by using an equivalent electrical circuit. Hayden model was chosen to analyze kiwifruit ripening process, which is composed of extracellular resistance, intracellular resistance and constant phase element representing the cytomembrane. Complex nonlinear least square (CNLS) method was used for fitting the EIS data and determining the parameters of Hayden model. The results indicated that the impedance magnitude of the expanded and control fruits decreased with increasing frequency, and impedance magnitude decreased with kiwifruit ripening only at low frequencies. It was obvious during the ripening process that the impedance of control fruits decreased more quickly than that of the expanded fruits. The phase angles of two types of kiwifruits increased sharply and then decreased quickly with the increase of frequency, and reached the maximum values at 12 kHz. Cole-cole plots of kiwifruit tissues presented the semicircles with different radiuses, which represent the characteristics of typical biological tissue. Cytomembrane impedances of expanded kiwifruits changed minimally during the ripening process of 15 days, but after 7 days, cytomembrane impedances of the control fruits dropped sharply. Extracellular fluid resistance of two types of kiwifruits initially decreased, then increased and finally decreased with ripening process. Extracellular fluid resistance of expanded fruits was larger than that of control fruits, and extracellular fluid resistance of control fruits dropped more quickly than that of expanded fruits after 7 days. Variation of intracellular fluid resistance of two types of kiwifruits was not obvious. Because plant growth regulator could alleviate the damage of cell membrane, EIS properties of two types of kiwifruits were different. So electrical impedance spectroscopy is useful for the identification of expended kiwifruits from untreated kiwifruits by impedance properties. Consequently use of EIS to analyze the ripening process provides a technological foundation for the detection of expanded fruits.