CAJ | 학술논문

本试验采用间接竞争酶联免疫分析法（ELISA法）测定了动物源性食品中红霉素的残留，结果显示：猪肉、猪肝、牛肉、鱼肉、虾肉、蜂蜜和牛奶中的红霉素检测限分别为4.13、8.23、0.78、3.75、7.94、3.09μg/kg和1.87μg/L，样品添加回收率范围为69.0%~107.5%，批内变异系数为5.7%~11.4%，批间变异系数为8.7%~14.0%；与硫氰酸红霉素的交叉反应率为114%，与琥乙红霉素的交叉反应率为67.4%，与其他药物的交叉反应率均小于0.1%。试验表明，采用ELISA法测定红霉素准确灵敏、重复性好、特异性高，适用于检测动物源性食品中的红霉素残留。
본시험채용간접경쟁매련면역분석법（ELISA법）측정료동물원성식품중홍매소적잔류，결과현시：저육、저간、우육、어육、하육、봉밀화우내중적홍매소검측한분별위4.13、8.23、0.78、3.75、7.94、3.09μg/kg화1.87μg/L，양품첨가회수솔범위위69.0%~107.5%，비내변이계수위5.7%~11.4%，비간변이계수위8.7%~14.0%；여류청산홍매소적교차반응솔위114%，여호을홍매소적교차반응솔위67.4%，여기타약물적교차반응솔균소우0.1%。시험표명，채용ELISA법측정홍매소준학령민、중복성호、특이성고，괄용우검측동물원성식품중적홍매소잔류。
An indirect competitive enzyme-linked immunosorbent assay(ELISA)was used to measure the residues of erythromycin in animal-derived food. The results showed that limits of detection in pork,pork liver,beef,fish,shrimp,honey and milk were 4.13,8.23,0.78,3.75,7.94,3.09μg/kg and 1.87μg/L respectively. Sample recoveries were 69.0%~107.5%, inter-assay coefficient variations were 5.7%~11.4%, and intra-assay coefficient variations were 8.7%~14.0%. The cross-reactivity towards erythromycin thiocyanate and erythromycin ethylsuccinate were 114% and 67.4% respectively. As for other drugs,the cross-reactivity was less than 0.1%. In a word,the sensitivity,accuracy,repeata-bility and specificity of ELISA method were satisfactory, it was suitable for measuring the residues of erythromycin in animal-derived food.