首都医科大学学报
首都醫科大學學報
수도의과대학학보
JOURNAL OF CAPITAL UNIVERSITY OF MEDICAL SCIENCES
2015年
1期
103-110
,共8页
赵春松%邹海强%闫晓明%陈凌%王佳茵%关云谦%张愚
趙春鬆%鄒海彊%閆曉明%陳凌%王佳茵%關雲謙%張愚
조춘송%추해강%염효명%진릉%왕가인%관운겸%장우
神经干细胞%凋亡%Bmi-1%癌基因
神經榦細胞%凋亡%Bmi-1%癌基因
신경간세포%조망%Bmi-1%암기인
neural stem cell%apoptosis%Bmi-1%oncogene
目的:探讨Bmi-1基因对人类胚胎纹状体来源神经干细胞(neural stem cell,NSC)凋亡的影响。方法提取16~18周自然流产胚胎脑组织纹状体的NSC,体外培养至3~5代,用慢病毒载体对其进行Bmi-1基因的RNA干扰和过表达回复。观察Bmi-1基因对体外NSC凋亡的影响。结果在感染后2 h和3 d两个时间点,Bmi-1基因的RNA干扰显著增加了体外培养的NSC的凋亡率。结论 Bmi-1基因对于体外培养的NSC具有抑制其凋亡的作用,在分析该基因对NSC增生、自我更新等生物学属性的影响时,应当将Bmi-1基因的抗凋亡作用考虑在内。
目的:探討Bmi-1基因對人類胚胎紋狀體來源神經榦細胞(neural stem cell,NSC)凋亡的影響。方法提取16~18週自然流產胚胎腦組織紋狀體的NSC,體外培養至3~5代,用慢病毒載體對其進行Bmi-1基因的RNA榦擾和過錶達迴複。觀察Bmi-1基因對體外NSC凋亡的影響。結果在感染後2 h和3 d兩箇時間點,Bmi-1基因的RNA榦擾顯著增加瞭體外培養的NSC的凋亡率。結論 Bmi-1基因對于體外培養的NSC具有抑製其凋亡的作用,在分析該基因對NSC增生、自我更新等生物學屬性的影響時,應噹將Bmi-1基因的抗凋亡作用攷慮在內。
목적:탐토Bmi-1기인대인류배태문상체래원신경간세포(neural stem cell,NSC)조망적영향。방법제취16~18주자연유산배태뇌조직문상체적NSC,체외배양지3~5대,용만병독재체대기진행Bmi-1기인적RNA간우화과표체회복。관찰Bmi-1기인대체외NSC조망적영향。결과재감염후2 h화3 d량개시간점,Bmi-1기인적RNA간우현저증가료체외배양적NSC적조망솔。결론 Bmi-1기인대우체외배양적NSC구유억제기조망적작용,재분석해기인대NSC증생、자아경신등생물학속성적영향시,응당장Bmi-1기인적항조망작용고필재내。
Objective To explore the effect of Bmi-1 on the apoptosis of human fetal striatum derived neural stem cell (NSC ). Methods The NSC was isolated from the striatum of 1 6-1 8 week fetus suffered from accidental spontaneous abortion,cultured and passaged 3-5 times in vitro.Bmi-1 RNA interference and rescue (over-expression of Bmi-1 )experiments were performed by lentivirus.The effects of Bmi-1 on the apoptosis of NSC were then observed.Results The Bmi-1 transcription of human striatum derived NSCs was decreased significantly by RNAi and was reversed by rescue experiments(overexpression of Bmi-1 ).Correspondingly,both at the time points of 2h and 3d after passaging,the RNAi of Bmi-1 increased the apoptosis significantly.Conclusion Given human fetal striatum derived NSCs,Bmi-1 inhibited their apoptosis in vitro.In the cases studying the effect of Bmi-1 on the self-renewal,proliferation of NSC, the anti-apoptosis effect of Bmi-1 should be taken into consideration.