CAJ | 학술논문

目的：建立软饮料中香兰素和乙基香兰素的高效液相色谱法检测方法。方法样品中的香兰素和乙基香兰素在酸性条件下经乙腈提取,利用盐析作用分层净化,提取液浓缩复溶解后,供液相色谱检测。检测波长308 nm,外标法定量。结果香兰素和乙基香兰素在0.5~50.0?g/mL浓度范围内线性关系良好,相关系数均大于0.9999。添加水平为5、30、63 mg/kg时,香兰素和乙基香兰素的平均回收率分别为98.4%和100.4%,96.7%和100.2%,97.8%和100.3%,相对标准偏差在3.53%~4.70%,检出限均为0.5 mg/kg。结论方法操作简便、快速,经济实用,适用性强,结果准确可靠,可用于样品批量快速检测。
목적：건립연음료중향란소화을기향란소적고효액상색보법검측방법。방법양품중적향란소화을기향란소재산성조건하경을정제취,이용염석작용분층정화,제취액농축복용해후,공액상색보검측。검측파장308 nm,외표법정량。결과향란소화을기향란소재0.5~50.0?g/mL농도범위내선성관계량호,상관계수균대우0.9999。첨가수평위5、30、63 mg/kg시,향란소화을기향란소적평균회수솔분별위98.4%화100.4%,96.7%화100.2%,97.8%화100.3%,상대표준편차재3.53%~4.70%,검출한균위0.5 mg/kg。결론방법조작간편、쾌속,경제실용,괄용성강,결과준학가고,가용우양품비량쾌속검측。
Objective To develop a method for determination of vanillin and ethyl vanillin in soft drink by high performance liquid chromatography (HPLC). Methods Vanillin and ethyl vanillin in sample were extracted with acetonitrile and 0.1%formic acid solution, and were cleaned up by salting out effect. After concentrated, the extraction was redissolved and determined by HPLC at 308 nm. The external standard method was used for quantitative analysis. Results The method showed a good linear over the range of 0.5~50.0?g/mL for vanillin and ethyl vanillin with correlation coefficients above 0.9999. The average recoveries were 98.4%and 100.4%, 96.7%and 100.2%, 97.8% and 100.3% at the spiked level of 5, 30, and 63 mg/kg, with relative standard deviation between 3.53%~4.70%. The limit of detection was 0.5 mg/kg. Conclusion The method is simple, rapid, economical and practical, and the result is accurate and reliable, which is suitable for batch sample determination.