CAJ | 학술논문

目的：研制肠侵袭性大肠埃希氏菌核酸标准样品,并对其均匀性和稳定性进行评价。方法本文研究了肠侵袭性大肠埃希氏菌核酸标准样品的关键制备技术和保存技术,定值技术和定值方式,开展均匀性和稳定性试验,并对食品中肠侵袭性大肠埃希氏菌核酸标准样品的不确定度进行确定；标准样品采用 PicoGreen DNA 分子荧光定量方法进行定值。多家单位合作定值,确定标准样品的标准值及其不确定度。结果标准样品均匀性标准不确定度为0.016%,不稳定标准差为0.0100,均匀性和稳定性试验结果表明,本文研制的标准样品均匀且稳定。结论研制出具有溯源性的肠侵袭性大肠埃希氏菌核酸标准样品并获得国家标准样品证书,可适用于肠侵袭性大肠埃希氏菌的检测和质量控制。
목적：연제장침습성대장애희씨균핵산표준양품,병대기균균성화은정성진행평개。방법본문연구료장침습성대장애희씨균핵산표준양품적관건제비기술화보존기술,정치기술화정치방식,개전균균성화은정성시험,병대식품중장침습성대장애희씨균핵산표준양품적불학정도진행학정；표준양품채용 PicoGreen DNA 분자형광정량방법진행정치。다가단위합작정치,학정표준양품적표준치급기불학정도。결과표준양품균균성표준불학정도위0.016%,불은정표준차위0.0100,균균성화은정성시험결과표명,본문연제적표준양품균균차은정。결론연제출구유소원성적장침습성대장애희씨균핵산표준양품병획득국가표준양품증서,가괄용우장침습성대장애희씨균적검측화질량공제。
Objective Enteroinvasive Escherichia Coli(EIEC) Molecular DNA Reference Material was developed, and uniformity and stability of test were evaluated. Methods The key preparation technology, saving technology, certified technique and mode for EIEC Molecular DNA Reference Material were researched, via uniformity and stability test. The uncertainty for EIEC Molecular DNA Reference Material was evaluated in food. The standard value was determined by PicoGreen DNA molecule fluorescence quantitative methods. Standard and uncertainty of reference material was determined by many units cooperation. Results Uniformity standard uncertainty of reference material was 0.016 %, unstable standard deviation was 0.0100. The result of uniformity and stability test showed that reference material developed in this study was uniform and stable. Conclusion EIEC Molecular DNA Reference Material with the traceability was developed, and certificate of national reference material was obtained. It could be used for quantitative detection and qualitative quality control of EIEC.