CAJ | 학술논문

目的：建立了一种快速检测进出口食品中转基因成分的方法。方法本实验采用DNA提取试剂盒对薯格中的DNA进行快速提取，接着用实时荧光PCR方法对其进行转基因成分和品系的鉴定。结果通过对食品标签进行初筛，发现其标识成分中含有未标明的转基因成分。进一步对所含转基因成分的品系进行鉴定，确定薯格的外包裹玉米粉中含有多种转基因成分，包括转基因玉米TC1507、NK603、MON810、59122、MON89034等5个品系。结论本文方法可以用于加工食品中转基因玉米成分及品系的定性检测，也可以作为常规 PCR定性方法的确证试验方法。
목적：건립료일충쾌속검측진출구식품중전기인성분적방법。방법본실험채용DNA제취시제합대서격중적DNA진행쾌속제취，접착용실시형광PCR방법대기진행전기인성분화품계적감정。결과통과대식품표첨진행초사，발현기표식성분중함유미표명적전기인성분。진일보대소함전기인성분적품계진행감정，학정서격적외포과옥미분중함유다충전기인성분，포괄전기인옥미TC1507、NK603、MON810、59122、MON89034등5개품계。결론본문방법가이용우가공식품중전기인옥미성분급품계적정성검측，야가이작위상규 PCR정성방법적학증시험방법。
Objective To establish a method for rapid detection of the import and export genetically modified foods. Methods The DNA of potato chips was quickly extract using DNA extraction kit, and the basic ingredients of the potato chips were identified by real-time PCR. Results Unmarked GMO was found in its identity component by preliminary screening of food labels. The potato chips with corn flour wrapped contained a variety of genetically modified ingredients, including transgenic strains TC1507, NK603, MON810, 59122, and MON89034, etc. Conclusion This method can be used for the qualitative detection of ingredients and strains of transgenic corn in processed food and as a confirmatory test methods qualitative for the conventional PCR methods.