CAJ | 학술논문

目的：研以基质辅助激光解析电离飞行时间质谱(matrix assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)方法检测大肠杆菌,并利用MALDI-TOF MS方法鉴别ESBLs菌株及聚类分析法区分不同分型的ESBLs菌株,探索MALDI-TOF MS方法鉴定大肠杆菌及ESBLs菌株的可行性。方法利用MALDI-TOF MS采集47株大肠杆菌分离株的质谱数据,生成肽指纹图谱,并利用biotyper软件进行菌种鉴定,同时研究其耐药性鉴定结果,并利用聚类分析法研究大肠杆菌 ESBLs 菌株,最终确定MALDI-TOF MS对大肠杆菌菌种鉴定及ESBLs菌株鉴别的可行性。结果 MALDI-TOF MS方法鉴定大肠杆菌的结果与传统方法及PCR方法检测结果均符合,经MALDI-TOF MS鉴定方法提示为ESBLs菌株的10株大肠杆菌中有9株为 ESBLs 菌株,且聚类分析法对 OXA 型 ESBLs 大肠杆菌具有较好的鉴别能力。结论MALDI-TOF MS方法可以快速、准确地鉴定大肠杆菌,对ESBLs菌株具有一定的筛选鉴别能力。
목적：연이기질보조격광해석전리비행시간질보(matrix assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)방법검측대장간균,병이용MALDI-TOF MS방법감별ESBLs균주급취류분석법구분불동분형적ESBLs균주,탐색MALDI-TOF MS방법감정대장간균급ESBLs균주적가행성。방법이용MALDI-TOF MS채집47주대장간균분리주적질보수거,생성태지문도보,병이용biotyper연건진행균충감정,동시연구기내약성감정결과,병이용취류분석법연구대장간균 ESBLs 균주,최종학정MALDI-TOF MS대대장간균균충감정급ESBLs균주감별적가행성。결과 MALDI-TOF MS방법감정대장간균적결과여전통방법급PCR방법검측결과균부합,경MALDI-TOF MS감정방법제시위ESBLs균주적10주대장간균중유9주위 ESBLs 균주,차취류분석법대 OXA 형 ESBLs 대장간균구유교호적감별능력。결론MALDI-TOF MS방법가이쾌속、준학지감정대장간균,대ESBLs균주구유일정적사선감별능력。
Objective The strains of Escherichia coli was tested by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). ESBLs strains were distinguished, and different type ESBLs strains was differentiated by clustering analysis. Feasibility of identification was explored by MALDI-TOF MS for E.coli and ESBLs strains. Methods Peptide mass fingerprints were obtained via collecting mass spectrometric data of 47 E.coli. Their species were identified by biotyper sortware, and drug resistance results were studied. ESBLs strains of E.coli were researched by clustering analysis. Feasibility of identification was confirmed by MALDI-TOF MS for E.coli and ESBLs strains. Results The identification results were coincident with conventional method and PCR method. There were 9 ESBLs stains in 10 E.coli that were prompted for ESBLs strains by MALDI-TOF MS. It had good distinguishing capability for OXA type ESBLs E.coli by cluster analysis. Conclusion E.coli strains could be rapidly, accurately identified by MALDI-TOF MS, and there was a certain screening capacity for ESBLs strains.