CAJ | 학술논문

目的：建立超声辅助溶剂萃取——直接测汞法测定海产品中甲基汞的检测方法,应用此方法检测黄鱼、鳕鱼、金枪鱼、扇贝、杂色蛤等海产品中甲基汞的含量。方法选择盐酸为萃取溶剂,通过优化萃取溶剂的浓度和提取条件,选择5 mol/L 盐酸作为溶剂,超声1 h,以二氯甲烷分两次萃取,再以水反萃取,应用测汞仪直接测定水相中甲基汞的含量。结果甲基汞标准曲线的线性相关系数大于0.997,检出限为0.75 ng/g。不同类型生物标准参考物质实际测定值与标准参考值相符,测定值的相对标准偏差小于4%。结论该方法操作简便、快速、准确,适用于鱼、贝类海产品中甲基汞的快速萃取分离和测定。不同种类样品中甲基汞污染程度不同,实验选取的样品中甲基汞的含量均未超过卫生限量,鳕鱼、金枪鱼中甲基汞含量较高。
목적：건립초성보조용제췌취——직접측홍법측정해산품중갑기홍적검측방법,응용차방법검측황어、설어、금창어、선패、잡색합등해산품중갑기홍적함량。방법선택염산위췌취용제,통과우화췌취용제적농도화제취조건,선택5 mol/L 염산작위용제,초성1 h,이이록갑완분량차췌취,재이수반췌취,응용측홍의직접측정수상중갑기홍적함량。결과갑기홍표준곡선적선성상관계수대우0.997,검출한위0.75 ng/g。불동류형생물표준삼고물질실제측정치여표준삼고치상부,측정치적상대표준편차소우4%。결론해방법조작간편、쾌속、준학,괄용우어、패류해산품중갑기홍적쾌속췌취분리화측정。불동충류양품중갑기홍오염정도불동,실험선취적양품중갑기홍적함량균미초과위생한량,설어、금창어중갑기홍함량교고。
Objective To establish a method for determination of methylmercury (MeHg) in seafood using a direct Hg analyzer (DMA-80), and apply this method to detect the content of MeHg in Yellow croaker, cod, tuna, scallops, and clams. Methods MeHg was separated from bio sample matrix with 5 mol/L hydrochloric acid by ultrasound assisted for 1 h. The extraction conditions were optimized. After back-extraction of MeHg from organic methylene chloride solvent to aqueous solution, MeHg was determined by the mercury analyzer. Results The standard curve of linear correlation coefficient was greater than 0.997, and the detection limit of MeHg was 0.75 ng/g. The experimental results of standard reference materials were in a good agreement with the certified values, and the relative standard deviation of the determined values were less than 4%. Conclusion The method takes the advantage of simplicity and accuracy, and can be applied for the analysis of MeHg in different seafoods. There is obvious difference of MeHg content levels in seafood samples. The MeHg content in experimental samples do not exceed the health limits, and MeHg content is higher in cod and tuna.