食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
1期
245-251
,共7页
弱酸性电位水%次氯酸钠%稀盐酸%单增李斯特菌%致死机制
弱痠性電位水%次氯痠鈉%稀鹽痠%單增李斯特菌%緻死機製
약산성전위수%차록산납%희염산%단증리사특균%치사궤제
slightly acidic electrolyzed water%sodium hypochlorite%hydrochloric acid%Listeria monocytogenes%sterilization mechanism
目的：研究弱酸性电位水(slightly acidic electrolyzed water, SAEW)对单增李斯特菌的致死机制,并与强碱性杀菌剂次氯酸钠(NaClO)和强酸性杀菌剂稀盐酸(HCl)进行比较。方法测定三种杀菌剂的杀菌效率及处理后胞内蛋白泄漏量、TTC-脱氢酶相对活性、Na+/K+-ATP酶活性和细菌超微结构的变化。结果30 mg/L SAEW和NaClO对Na+/K+-ATP酶和TTC-脱氢酶相对活性的抑制无显著性差异,但其显著高于稀HCl组。SAEW处理单增李斯特菌1 min 后所导致的蛋白质泄漏量(0.35 mg/mL)显著高于 NaClO 和稀 HCl 处理组(分别为0.16 mg/mL和0.11 mg/mL)。SAEW、NaClO、稀HCl对单增李斯特菌的细胞超微结构都造成了明显的损伤,细胞质凝集,透电子区扩大。其中稀HCl对超微结构的损伤最大,说明强酸性环境对细胞形态结构有很大的影响。结论 SAEW 处理对单增李斯特菌细胞质造成了一定程度的损伤,抑制了细胞呼吸作用,改变了细胞膜的通透性,降低了细胞膜上的 Na+/K+-ATP 酶活性,导致细胞内大分子物质泄漏而最终导致细胞死亡。SAEW 杀菌机制的初步研究为未来SAEW的杀菌研究和应用前景奠定基础。
目的：研究弱痠性電位水(slightly acidic electrolyzed water, SAEW)對單增李斯特菌的緻死機製,併與彊堿性殺菌劑次氯痠鈉(NaClO)和彊痠性殺菌劑稀鹽痠(HCl)進行比較。方法測定三種殺菌劑的殺菌效率及處理後胞內蛋白洩漏量、TTC-脫氫酶相對活性、Na+/K+-ATP酶活性和細菌超微結構的變化。結果30 mg/L SAEW和NaClO對Na+/K+-ATP酶和TTC-脫氫酶相對活性的抑製無顯著性差異,但其顯著高于稀HCl組。SAEW處理單增李斯特菌1 min 後所導緻的蛋白質洩漏量(0.35 mg/mL)顯著高于 NaClO 和稀 HCl 處理組(分彆為0.16 mg/mL和0.11 mg/mL)。SAEW、NaClO、稀HCl對單增李斯特菌的細胞超微結構都造成瞭明顯的損傷,細胞質凝集,透電子區擴大。其中稀HCl對超微結構的損傷最大,說明彊痠性環境對細胞形態結構有很大的影響。結論 SAEW 處理對單增李斯特菌細胞質造成瞭一定程度的損傷,抑製瞭細胞呼吸作用,改變瞭細胞膜的通透性,降低瞭細胞膜上的 Na+/K+-ATP 酶活性,導緻細胞內大分子物質洩漏而最終導緻細胞死亡。SAEW 殺菌機製的初步研究為未來SAEW的殺菌研究和應用前景奠定基礎。
목적：연구약산성전위수(slightly acidic electrolyzed water, SAEW)대단증리사특균적치사궤제,병여강감성살균제차록산납(NaClO)화강산성살균제희염산(HCl)진행비교。방법측정삼충살균제적살균효솔급처리후포내단백설루량、TTC-탈경매상대활성、Na+/K+-ATP매활성화세균초미결구적변화。결과30 mg/L SAEW화NaClO대Na+/K+-ATP매화TTC-탈경매상대활성적억제무현저성차이,단기현저고우희HCl조。SAEW처리단증리사특균1 min 후소도치적단백질설루량(0.35 mg/mL)현저고우 NaClO 화희 HCl 처리조(분별위0.16 mg/mL화0.11 mg/mL)。SAEW、NaClO、희HCl대단증리사특균적세포초미결구도조성료명현적손상,세포질응집,투전자구확대。기중희HCl대초미결구적손상최대,설명강산성배경대세포형태결구유흔대적영향。결론 SAEW 처리대단증리사특균세포질조성료일정정도적손상,억제료세포호흡작용,개변료세포막적통투성,강저료세포막상적 Na+/K+-ATP 매활성,도치세포내대분자물질설루이최종도치세포사망。SAEW 살균궤제적초보연구위미래SAEW적살균연구화응용전경전정기출。
Objective To research the lethal injury mechanism of slightly acidic electrolyzed water on Listeria monocytogenes in comparison with traditional disinfectants such as sodium hypochlorite (NaClO) and hydrochloric acid (HCl). Methods The inactivation of L. monocytogenes by SAEW, NaClO, and HCl was determined while the disinfection mechanism was investigated by measuring changes to intracellular protein leakage, TTC-dehydrogenase relative activity, Na+/K+-ATPase activity and cellular ultrastructure as indicators. Results 30 mg/L SAEW and NaClO treatments had no significant difference of inhibition on the activity of Na+/K+-ATPase and relative activity of TTC-dehydrogenase, but they were significantly higher than that by dilute hydrochloric acid treatment. However, the protein leakage from L. monocytogenes caused by SAEW in 1 min (0.35 mg/mL) was significantly higher than that of NaClO and HCl treatment (0.16 mg/mL and 0.11 mg/mL). SAEW, NaClO and dilute HCl caused remarkably damages on the ultrastructure of L. monocytogenes, partly cytoplasm agglutination and expansion of electron transmission. The maximum damages were caused by dilute HCl which illustrated that strong acid has a great impact on cell morphology. Conclusion SAEW partly disrupted the cytoplasm of cell, inhibited cellular respiration, changed the permeability of cell membrane, induced intracellular macromolecules leakage and eventually lead to cell death. This preliminary research which focused on the sterilization mechanism of SAEW had laid the foundation for the future investigations and application of SAEW.
