CAJ | 학술논문

目的：建立实验室生物合成15N稳定同位素标记集胞藻的最优培养条件，并驯化得到高丰度15N集胞藻藻种。方法对培养温度、光照及pH值等条件进行优化，优选出最佳培养工艺。将培养基中的14N氮源替换为15N氮源，优化配方，使用同位素比质谱仪测量藻体中15N、14N同位素组成。结果实验室培养条件选定为培养温度25℃、光照强度2000 lux、初始pH值为7时，集胞藻生长良好。将配方中硝酸钠浓度设定为1.5 g/L时，产品丰度最大。驯化得到高丰度15N-集胞藻藻种。结论本试验优化的培养工艺和配方，适宜15N稳定同位素标记集胞藻生长。得到的藻种产品丰度高，生产成本低。
목적：건립실험실생물합성15N은정동위소표기집포조적최우배양조건，병순화득도고봉도15N집포조조충。방법대배양온도、광조급pH치등조건진행우화，우선출최가배양공예。장배양기중적14N담원체환위15N담원，우화배방，사용동위소비질보의측량조체중15N、14N동위소조성。결과실험실배양조건선정위배양온도25℃、광조강도2000 lux、초시pH치위7시，집포조생장량호。장배방중초산납농도설정위1.5 g/L시，산품봉도최대。순화득도고봉도15N-집포조조충。결론본시험우화적배양공예화배방，괄의15N은정동위소표기집포조생장。득도적조충산품봉도고，생산성본저。
Objective To establish the optimal culture conditions of biosynthesis 15N stable isotope labeled Synechocystis, and to get high abundance 15N Synechocystis sp. strains. Methods The culture temperature, shine intensity and pH for Synechocystis sp. were studied in this paper. 15N nitrogen was used to replace 14N nitrogen in the medium. Isotope composition of 15N, 14N was measured by isotope ratio mass spectrometer (IRMS). Results The optimal medium conditions for Synechocystis sp. strain based on BG-11 medium were determined as follows:the NaNO3 concentration was 1.5 g/L (the rest of the components were the same as BG-11 medium). The optimal culture conditions were as follows: temperature of 25 ℃, light intensity of 2000 lux and initial pH of 7 in the room. Conclusion This paper built an academic foundation for achieving high density and amplificatory culture of Synechocystis sp. PCC6803.