肉类研究
肉類研究
육류연구
MEAT RESEARCH
2014年
12期
17-20
,共4页
潘晓倩%成晓瑜%张顺亮%乔晓玲%陈文华
潘曉倩%成曉瑜%張順亮%喬曉玲%陳文華
반효천%성효유%장순량%교효령%진문화
抗菌肽%抑菌活性%测定方法
抗菌肽%抑菌活性%測定方法
항균태%억균활성%측정방법
antimicrobial peptide%antimicrobial activity%assay
目的：比较5种抗菌肽活性测定方法。方法：以金黄色葡萄球菌、溶血性葡萄球菌和枯草芽孢杆菌为实验菌株，研究牛津杯法、打孔法、滤纸片法、酶标比浊法和活菌计数法对不同质量浓度的抗菌肽抑菌活性评价的比较。结果：3种琼脂扩散定性抑菌实验中，打孔法灵敏度高，抑菌圈清晰规则，效果最好；2种定量抑菌实验中，活菌计数法结果判定更直观，灵敏度高，效果最好。结论：抗菌肽抑菌活性研究过程中，选用打孔法定性与活菌计数法定量相结合的方法，能够更全面、准确地反应其抑菌活性。
目的：比較5種抗菌肽活性測定方法。方法：以金黃色葡萄毬菌、溶血性葡萄毬菌和枯草芽孢桿菌為實驗菌株，研究牛津杯法、打孔法、濾紙片法、酶標比濁法和活菌計數法對不同質量濃度的抗菌肽抑菌活性評價的比較。結果：3種瓊脂擴散定性抑菌實驗中，打孔法靈敏度高，抑菌圈清晰規則，效果最好；2種定量抑菌實驗中，活菌計數法結果判定更直觀，靈敏度高，效果最好。結論：抗菌肽抑菌活性研究過程中，選用打孔法定性與活菌計數法定量相結閤的方法，能夠更全麵、準確地反應其抑菌活性。
목적：비교5충항균태활성측정방법。방법：이금황색포도구균、용혈성포도구균화고초아포간균위실험균주，연구우진배법、타공법、려지편법、매표비탁법화활균계수법대불동질량농도적항균태억균활성평개적비교。결과：3충경지확산정성억균실험중，타공법령민도고，억균권청석규칙，효과최호；2충정량억균실험중，활균계수법결과판정경직관，령민도고，효과최호。결론：항균태억균활성연구과정중，선용타공법정성여활균계수법정량상결합적방법，능구경전면、준학지반응기억균활성。
Objective:To comparatively evaluate the performance of several antimicrobial peptide activity assays. Methods:Using Staphylococcus aureus, Staphylococcus haemolyticus and Bacillus subtilis as experimental strains, the antimicrobial activity of the antimicrobial peptide nisin at different concentrations was determined by Oxford cup method, punching method, filter paper method, microtiter plate assay and viable count method, respectively. Results: Among three agar-diffusion methods, punching method was the most sensitive, which provided clear and regular inhibition rings. Our comparison of two quantitative activity assays indicated that viable count method, showing high sensitivity and intuitive results, was better than microtiter plate assay. Conclusion: Combined application of punching method and viable count method allows comprehensive and accurate measurement of antimicrobial peptide activity.
