医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
5期
230-230
,共1页
广州管圆线虫%幼虫%中性红%台盼蓝%美蓝
廣州管圓線蟲%幼蟲%中性紅%檯盼藍%美藍
엄주관원선충%유충%중성홍%태반람%미람
Angiostrongylus cantonensis%Larvae%Neutral red%Trypan blue%Methylene blue
目的寻找快速鉴别广州管圆线虫死活的染色方法。方法用中性红、台盼蓝和美蓝三种染液对活或死广州管圆线虫幼虫进行染色。结果中性红染液和美蓝染液可以使死亡的广州管圆线虫幼虫染色，活虫不染色且活力不受染色剂影响；台盼蓝染液对广州管圆线虫死、活幼虫均不染色。结论中性红和美蓝染液可快速鉴别广州管圆线虫幼虫的死活。
目的尋找快速鑒彆廣州管圓線蟲死活的染色方法。方法用中性紅、檯盼藍和美藍三種染液對活或死廣州管圓線蟲幼蟲進行染色。結果中性紅染液和美藍染液可以使死亡的廣州管圓線蟲幼蟲染色，活蟲不染色且活力不受染色劑影響；檯盼藍染液對廣州管圓線蟲死、活幼蟲均不染色。結論中性紅和美藍染液可快速鑒彆廣州管圓線蟲幼蟲的死活。
목적심조쾌속감별엄주관원선충사활적염색방법。방법용중성홍、태반람화미람삼충염액대활혹사엄주관원선충유충진행염색。결과중성홍염액화미람염액가이사사망적엄주관원선충유충염색，활충불염색차활력불수염색제영향；태반람염액대엄주관원선충사、활유충균불염색。결론중성홍화미람염액가쾌속감별엄주관원선충유충적사활。
Objective To exploretheprompt methods indeterminationof viabilityof the larvae of Angiostrongylus cantonensis.Methods The larvae of Angiostrongylus cantonensis larvae were stained with neutral red、trypan blue or methylene blue.Results The dead Angiostrongylus cantonensis larvae were observed when stained with neutral red 、methylene blue.The vital larvae were unstained with al three stains.Trypan blue do not staind the larvae neither live or dead.Conclusion Both neutral red and methylene blue staining are prompt method in determination of viability of the larvae of Angiostrongylus cantonensis.
