CAJ | 학술논문

目的：探讨头孢曲松钠（Ceftriaxone，Cef）对慢性神经病理性痛过敏及GLT-1表达的影响。方法雄性SD大鼠90只，随机分为Sham组、CCI 14 d组、Cef预防组、 Cef治疗组，后2组设腹腔注射NS组为对照，在不同时间点测定热缩足反射潜伏期；另设CCI 1 d、4 d、7 d组。应用免疫组化观察不同时间点脊髓后角GLT-1表达的变化。结果CCI诱导大鼠产生了热痛敏，腹腔注射NS对CCI诱导的热痛敏无影响。 Cef预防组大鼠CCI侧后肢热缩足反射潜伏期于CCI后第5天、第7天明显延长（ P ＜0°．05）；Cef 治疗组大鼠CCI侧后肢热缩足反射潜伏期在术后第11天、第14天明显延长（ P ＜0．05）。免疫组化检测发现，Cef预防组和Cef治疗组分别抑制和逆转了GLT-1在CCI后期表达的降低。结论 Cef可通过上调GLT-1的表达对慢性神经病理性痛起到预防和治疗作用。
목적：탐토두포곡송납（Ceftriaxone，Cef）대만성신경병이성통과민급GLT-1표체적영향。방법웅성SD대서90지，수궤분위Sham조、CCI 14 d조、Cef예방조、 Cef치료조，후2조설복강주사NS조위대조，재불동시간점측정열축족반사잠복기；령설CCI 1 d、4 d、7 d조。응용면역조화관찰불동시간점척수후각GLT-1표체적변화。결과CCI유도대서산생료열통민，복강주사NS대CCI유도적열통민무영향。 Cef예방조대서CCI측후지열축족반사잠복기우CCI후제5천、제7천명현연장（ P ＜0°．05）；Cef 치료조대서CCI측후지열축족반사잠복기재술후제11천、제14천명현연장（ P ＜0．05）。면역조화검측발현，Cef예방조화Cef치료조분별억제화역전료GLT-1재CCI후기표체적강저。결론 Cef가통과상조GLT-1적표체대만성신경병이성통기도예방화치료작용。
Objective To investigate the effects of ( Ceftriaxone ,Cef) on chronic neuropathic pain and the expression of GLT-1 in rats.Methods Ninety male Spague-Dawley rats were randomly divided into sham-operation group, CCI 14d group,Cef prevention group,Cef therapy group,at the same time,the two groups with intraperitoneal injection with NS were designed as control groups for the last two groups .The thermal withdrawal latency was measured in different time points , in addition,CCI 1d,CCI 4d and CCI 7d groups were designed to observe dynamic change of GLT-1 expression after CCI.The changes of GLT-1 expression in dorsal horn of spinal cord were detected by immunohistochemistry .Results The thermal hyperalgesia in rats was induced by chronic crushing injury (CCI),however,intraperitoneal injection with NS had no effect on thermal hyperalgesia induced by CCI .The thermal withdrawal latency was obviously prolonged in Cef prevention group on the 5th day,the 7th day after CCI( P <0.05),moreover,which in Cef therapy group was significantly prolonged on the 11th day, 14th day after CCI( P <0.05).The results by immunohistochemisty showed that Cef prevention group and Cef therapy group inhibited and reversed the down-regulation of GLT-1 expression at CCI later period .Conclusion The Cef can prevent and treat chronic neuropathic pain by up-regulating the expression of GLT-1.