CAJ | 학술논문

为了建立简便、快速筛选高效、低毒的新型抗氧化药物的方法，本研究采用快原子轰击离子化质谱( FAB-MS)对酚类化合物抗氧化作用强弱进行了研究。考察了基质、轰击时间和样本浓度对FAB-MS 谱中分子离子(M+·)相对强度的影响，研究了黄酮类化合物在FAB-MS谱中的I(M+·)/I([M+H]+)比值与其抗氧化活性的相关性。对12种酚类化合物的抗氧化活性进行了预测与评价，并与硫代巴比妥酸法( TBA)检测的脂质过氧化抑制率进行比较，验证 FAB-MS 方法的可靠性。结果表明，由 FAB-MS 获得的 I ( M+·)/I([ M+H]+)比值能较好地反映酚类化合物抗氧化作用的强弱，可以作为抗氧化剂的体外筛选或预测方法，有助于加速新型抗氧化药物的发现。
위료건립간편、쾌속사선고효、저독적신형항양화약물적방법，본연구채용쾌원자굉격리자화질보( FAB-MS)대분류화합물항양화작용강약진행료연구。고찰료기질、굉격시간화양본농도대FAB-MS 보중분자리자(M+·)상대강도적영향，연구료황동류화합물재FAB-MS보중적I(M+·)/I([M+H]+)비치여기항양화활성적상관성。대12충분류화합물적항양화활성진행료예측여평개，병여류대파비타산법( TBA)검측적지질과양화억제솔진행비교，험증 FAB-MS 방법적가고성。결과표명，유 FAB-MS 획득적 I ( M+·)/I([ M+H]+)비치능교호지반영분류화합물항양화작용적강약，가이작위항양화제적체외사선혹예측방법，유조우가속신형항양화약물적발현。
A fast atom bombardment mass spectrometric method to predict and detect the antioxidant ability of phenolic compounds was developed to accelerate the pace of finding the antioxidant with higher effect and low toxicity. The effect of experimental conditions on the relative peak intensity ratio of M+· ion to [ M+H]+ion in the FAB mass spectra of the compound was investigated, including matrix, scan time and concentration. The correlation of antioxidant activity with the I ( M+· )/I ( [ M+H ]+) value of flavonoids obtained in FAB mass spectra was studied. Then the antioxidant activity of 12 phenolic compounds was predicted using the above method and the results were compared with those obtained from thiobarbituric acid ( TBA) method. The results show that the I( M+· )/I( [ M+H]+) value of the phenolic compound obtained from FAB mass spectra could reflect their antioxidant activity, which could help to accelerate the development of the antioxidant drug.