CAJ | 학술논문

还原容量( RC)是衡量水溶性有机物( DOM)还原特性的重要指标。分别取未腐熟和腐熟后筛分的堆肥样品提取DOM并分离亲水性组分( HyI)作为电子供体及电子穿梭体,以3种不同形态的铁作电子受体测定其还原容量。结果表明,对于3种形态的电子受体Fe2(SO4)3, Fe(NO3)3和柠檬酸铁(FeCit),腐熟筛分后HyI的还原容量值分别为15.88,13.41和51.45 mmol e -/mol C,均大于未腐熟阶段的HyI还原容量值13.45,11.77和43.16 mmol e -/mol C。同时还发现不同电子受体对HyI的还原能力影响明显,FeCit条件下测得的RC值明显高于在Fe2(SO4)3和Fe(NO3)3条件下的RC值。而且HyI的微生物还原容量低于本底还原容量。通过紫外-可见光谱特征值(包括SUVA254和SUVA280)、特征值比值(包括A250/A365和A465/A665)及面积积分比值分析发现,芳香族、不饱和共轭双键结构、芳化度和有机质分子量都会对HyI氧化还原能力产生影响；结合三维荧光光谱体积积分值及百分比分析发现,类腐殖质(包括类富里酸和类胡敏酸)相对含量是HyI还原容量主要影响因素。本研究为科学表征亲水性组分的氧化还原特性、揭示其在堆肥体系污染物降解转化中的作用提供了科学依据。
환원용량( RC)시형량수용성유궤물( DOM)환원특성적중요지표。분별취미부숙화부숙후사분적퇴비양품제취DOM병분리친수성조분( HyI)작위전자공체급전자천사체,이3충불동형태적철작전자수체측정기환원용량。결과표명,대우3충형태적전자수체Fe2(SO4)3, Fe(NO3)3화저몽산철(FeCit),부숙사분후HyI적환원용량치분별위15.88,13.41화51.45 mmol e -/mol C,균대우미부숙계단적HyI환원용량치13.45,11.77화43.16 mmol e -/mol C。동시환발현불동전자수체대HyI적환원능력영향명현,FeCit조건하측득적RC치명현고우재Fe2(SO4)3화Fe(NO3)3조건하적RC치。이차HyI적미생물환원용량저우본저환원용량。통과자외-가견광보특정치(포괄SUVA254화SUVA280)、특정치비치(포괄A250/A365화A465/A665)급면적적분비치분석발현,방향족、불포화공액쌍건결구、방화도화유궤질분자량도회대HyI양화환원능력산생영향；결합삼유형광광보체적적분치급백분비분석발현,류부식질(포괄류부리산화류호민산)상대함량시HyI환원용량주요영향인소。본연구위과학표정친수성조분적양화환원특성、게시기재퇴비체계오염물강해전화중적작용제공료과학의거。
Reduction capacity ( RC ) is an important index to evaluate the redox ability of dissolved organic matter. In order to determine the RC, hydrophilic organic fractions ( HyI ) isolated from dissolved organic matter extracted from the uncomposted and composted samples were used as electron donators and mediators, and three kinds of irons were chosen as electron acceptors. The results showed that, the RC values from the composted sample were 15. 88, 13. 41 and 51. 45 mmol e -/mol C for the electron acceptors Fe2(SO4)3, Fe(NO3)3 and FeCit, respectively, which were higher than the corresponding values (13. 45, 11. 77 and 43. 16 mmol e-/mol C) from the uncomposted sample. The electron acceptor type shows a dramatic influence on the RC value of HyI. The RC value determined by FeCit was obviously higher than that measured using Fe2( SO4 ) 3 and Fe( NO3 ) 3 , and the microbial reducing capacity of the HyI was lower than the corresponding native reducing capacity. By analyzing the special absorbencies ( SUVA254 and SUVA280 ) , absorbance ratios ( A2/A3 and A4/A6 ) and integrated area from UV-vis spectra, it can be found that the RC was affected by aromatic degree, unsaturated conjugated structure, and molecular weight. Excitation-emission matrix spectra coupled with regional integration analysis showed that the relative content of humic-like substances ( humic-like acids and fulvic-like acids) was the main factor influencing the RC value of HyI. The results obtained can be used to characterize the redox properties of HyI, and reveal its role in the transformation and degradation of pollutants during composting.