CAJ | 학술논문

目的：考察黄芩中总黄酮与黄芩苷最佳的提取纯化工艺。方法采用紫外分光光度法测定黄芩总黄酮含量，高效液相色谱法测定黄芩苷含量，对不同提取方法、提取时间、提取次数、加水量等进行单因素及正交试验设计考察，确定最佳提取工艺。结果确定的最佳提取工艺为：水加热回流，提取时间1.5 h，提取次数为3次，加水量为8倍量。结论优选出的提取工艺稳定、合理、可行。
목적：고찰황금중총황동여황금감최가적제취순화공예。방법채용자외분광광도법측정황금총황동함량，고효액상색보법측정황금감함량，대불동제취방법、제취시간、제취차수、가수량등진행단인소급정교시험설계고찰，학정최가제취공예。결과학정적최가제취공예위：수가열회류，제취시간1.5 h，제취차수위3차，가수량위8배량。결론우선출적제취공예은정、합리、가행。
Objective To investigate the optimum extraction of total flavonoids and baicalin in baical skullcap root. Methods Ultraviolet spectrophotometry was used to detect the content of total flavonoids, HPLC was used to detect the content of baicalin. With the test of extracting method, time, frequency, and adding water volume, the optimum extracting craft was determined. Results The best craft for extraction was:heating water for recirculation, setting extracting time for 1.5 h, extracting frequency for 3 times, and water volume for 8 times of baical skullcap root. Conclusion The optimized process for extraction are stable, reasonable and feasible.