CAJ | 학술논문

目的：探讨葡萄糖酸锌与丹参注射液联合应用对噪声暴露大鼠血脂水平的影响。方法50只SD雌性大鼠按随机数字表法分为对照组、模型组、葡萄糖酸锌组、丹参注射液组、葡萄糖酸锌＋丹参注射液组（联合组），各10只。葡萄糖酸锌组灌胃葡萄糖酸锌8～10 mg/(kg?d)，丹参注射液组腹腔注射丹参注射液6 mg/(kg?d)，联合组葡萄糖酸锌8～10 mg/(kg?d)灌胃并腹腔注射丹参注射液6 mg/(kg?d)，共17 d。给药第10天时，除对照组外，各组均连续暴露于高频稳态噪声7 d（4000 Hz，95 dB，3 h/d）。检测血清总胆固醇（total cholesterol, TC）、三酰甘油（triglyceride, TG）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol, HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol, LDL-C）水平。结果与对照组比较，模型组大鼠血清 TC[(1.88±0.17)mmol/L 比(1.55±0.09)mmol/L]、TG[(1.45±0.38)mmol/L比(1.01±0.27)mmol/L]、LDL-C[(0.29±0.04)mmol/L比(0.23±0.03)mmol/L]水平显著高于对照组（ P＜0.01或 P＜0.05），而 HDL-C 水平显著低于对照组[(0.55±0.08)mmol/L 比(0.62±0.06)mmol/L；P＜0.01]。葡萄糖酸锌组血清TC[(1.71±0.11)mmol/L比(1.88±0.17)mmol/L]显著低于模型组（P＜0.05），丹参注射液组血清TC[(1.73±0.21)mmol/L比(1.88±0.17)mmol/L]、LDL-C[(0.25±0.03)mmol/L比(0.29±0.04)mmol/L]水平显著低于模型组（P均＜0.05），联合组血清TC[(1.57±0.21)mmol/L比(1.88±0.17)mmol/L]、TG[(0.84±0.40)mmol/L 比(1.45±0.38)mmol/L]、LDL-C[(0.24±0.05)mmol/L 比(0.29±0.04)mmol/L]水平显著低于模型组（ P＜0.01或 P＜0.05）， HDL-C 水平显著高于模型组[(0.61±0.07)mmol/L比(0.55±0.08)mmol/L；P＜0.05]。结论噪声暴露可引起大鼠血脂异常，葡萄糖酸锌和丹参注射液对噪声暴露大鼠血脂有调节作用，且两者联合应用优于单独用药。
목적：탐토포도당산자여단삼주사액연합응용대조성폭로대서혈지수평적영향。방법50지SD자성대서안수궤수자표법분위대조조、모형조、포도당산자조、단삼주사액조、포도당산자＋단삼주사액조（연합조），각10지。포도당산자조관위포도당산자8～10 mg/(kg?d)，단삼주사액조복강주사단삼주사액6 mg/(kg?d)，연합조포도당산자8～10 mg/(kg?d)관위병복강주사단삼주사액6 mg/(kg?d)，공17 d。급약제10천시，제대조조외，각조균련속폭로우고빈은태조성7 d（4000 Hz，95 dB，3 h/d）。검측혈청총담고순（total cholesterol, TC）、삼선감유（triglyceride, TG）、고밀도지단백담고순（high density lipoprotein cholesterol, HDL-C）、저밀도지단백담고순（low density lipoprotein cholesterol, LDL-C）수평。결과여대조조비교，모형조대서혈청 TC[(1.88±0.17)mmol/L 비(1.55±0.09)mmol/L]、TG[(1.45±0.38)mmol/L비(1.01±0.27)mmol/L]、LDL-C[(0.29±0.04)mmol/L비(0.23±0.03)mmol/L]수평현저고우대조조（ P＜0.01혹 P＜0.05），이 HDL-C 수평현저저우대조조[(0.55±0.08)mmol/L 비(0.62±0.06)mmol/L；P＜0.01]。포도당산자조혈청TC[(1.71±0.11)mmol/L비(1.88±0.17)mmol/L]현저저우모형조（P＜0.05），단삼주사액조혈청TC[(1.73±0.21)mmol/L비(1.88±0.17)mmol/L]、LDL-C[(0.25±0.03)mmol/L비(0.29±0.04)mmol/L]수평현저저우모형조（P균＜0.05），연합조혈청TC[(1.57±0.21)mmol/L비(1.88±0.17)mmol/L]、TG[(0.84±0.40)mmol/L 비(1.45±0.38)mmol/L]、LDL-C[(0.24±0.05)mmol/L 비(0.29±0.04)mmol/L]수평현저저우모형조（ P＜0.01혹 P＜0.05）， HDL-C 수평현저고우모형조[(0.61±0.07)mmol/L비(0.55±0.08)mmol/L；P＜0.05]。결론조성폭로가인기대서혈지이상，포도당산자화단삼주사액대조성폭로대서혈지유조절작용，차량자연합응용우우단독용약。
Objective To investigate the effects of zinc gluconate combined with Danshen injection on the plasma lipid level following exposure to noise in rats. Methods Fifty female SD rats were randomly divided into a control group, a noise exposure group (model group), a zinc gluconate + noise exposure group (zinc gluconate group), a Danshen injection+noise exposure group (Danshen injection group) and a zinc gluconate+Danshen injection+noise exposure group (combination group), 10 rats in each group. The zinc gluconate group received intragastric administration of zinc gluconate 8-10 mg/(kg?d), the Danshen injection group received intraperitoneal injection of Danshen injection 6 mg/(kg?d), the combination group received intragastric administration of zinc gluconate 8-10 mg/(kg?d) and intraperitoneal injection of Danshen injection 6 mg/(kg?d). On the day 10, except in the control group, the rats in the rest of the groups were continuously exposed to high frequency steady noise for 7 days (4 000 Hz, 95 dB, 3 h/d). The serum levels of total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were detected. Results The serum levels of TC (1.88 ± 0.17 mmol/L vs. 1.55 ±0.09 mmol/L), TG (1.45 ± 0.38 mmol/L vs. 1.01 ± 0.27 mmol/L), LDL-C (0.29 ± 0.04 mmol/L vs. 0.23 ± 0.03 mmol/L) in the model group were significantly lower than those in the control group (P<0.01 or P<0.05), while the serum HDL-C level in the model group were significantly lower than that in the control group (0.55 ± 0.08 mmol/L vs. 0.62 ±0.06 mmol/L;P<0.01). The serum TC level in the zinc gluconate group was significantly lower than that in the model group (1.71 ± 0.11 mmol/L vs.1.88 ± 0.17 mmol/L;P<0.05). The serum levels of TC (1.73 ± 0.21 mmol/L vs. 1.88 ± 0.17 mmol/L) and LDL-C (0.25 ± 0.03 mmol/L vs. 0.29 ± 0.04 mmol/L) in the Danshen injection group were significantly lower than those in the model group(all P<0.05). The serum levels of TC (1.57 ± 0.21 mmol/L vs. 1.88 ± 0.17 mmol/L), TG (0.84 ±0.40 mmol/L vs. 1.45 ± 0.38 mmol/L) and LDL-C (0.24 ± 0.05 mmol/L vs. 0.29 ± 0.04 mmol/L) in the combination group were significantly lower than those in the model group (P<0.01 or P<0.05), and the serum HDL-C level (0.61 ± 0.07 mmol/L vs. 0.55 ± 0.08 mmol/L) in the combination group was significantly higher than that in the model group (P<0.05). Conclusions Noise exposure may induce dyslipidemias in rats. Zinc gluconate and Danshen injection can regulate the plasma lipid level following exposure to noise in rats, and combination of zinc gluconate and Danshen injection may be superior to zinc gluconate or Danshen injection alone.