CAJ | 학술논문

目的：通过对比研究不同浓度斑蝥素（CA）和去甲斑蝥素（NCTD）对体外人膀胱肿瘤EJ、T24和鳞状细胞癌细胞（BSCC）增殖的抑制作用，明确药物效果、最佳药物浓度其抗肿瘤细胞增殖的机制。方法使用不同浓度的CA和NCTD与肿瘤细胞共同培养，MTT实验计算细胞生长抑制率，进而选出抑制肿瘤细胞的有效药物及其最佳浓度。选用最佳浓度的最佳药物与肿瘤细胞共同培养，不同时间收集细胞，电子显微镜及激光共聚焦显微镜观察细胞形态。结果 CA在体外对人膀胱肿瘤EJ、T24和BSCC细胞增殖有明显抑制作用，NCTD对于EJ和T24细胞有增殖有明显抑制作用，对BSCC细胞增殖的无明显抑制作用。在浓度小于40μmol/L、作用时间12～24 h的情况下，CA对EJ和T24细胞的增殖抑制作用具有明显的时间-效应和剂量-效应关系，24 h时药物半数抑制浓度（IC50）为20μmol/L；在浓度小于60μmol/L、作用时间24～48 h情况下，CA对BSCC的抑制作用具有明显的时间-效应和剂量-效应关系，48 h时药物IC50为60μmol/L。在浓度小于80μmol/L、作用时间24～48 h情况下，NCTD对EJ和T24细胞的增殖抑制作用具有明显的时间-效应和剂量-效应关系；24 h时药物IC50为60μmol/L。激光共聚焦扫描显微镜及电子显微镜观察证实CA和NCTD通过诱导膀胱肿瘤细胞凋亡进而抑制其增殖。结论 CA及NCTD均具有较好的抑制常见膀胱肿细胞增殖的作用，有望用于浅表膀胱肿瘤电切术后的膀胱灌注化疗；且CA对BSCC细胞增殖具有较好的抑制作用，有望用于膀胱肿瘤电切标本中伴发 BSCC患者的膀胱灌注化疗。
목적：통과대비연구불동농도반모소（CA）화거갑반모소（NCTD）대체외인방광종류EJ、T24화린상세포암세포（BSCC）증식적억제작용，명학약물효과、최가약물농도기항종류세포증식적궤제。방법사용불동농도적CA화NCTD여종류세포공동배양，MTT실험계산세포생장억제솔，진이선출억제종류세포적유효약물급기최가농도。선용최가농도적최가약물여종류세포공동배양，불동시간수집세포，전자현미경급격광공취초현미경관찰세포형태。결과 CA재체외대인방광종류EJ、T24화BSCC세포증식유명현억제작용，NCTD대우EJ화T24세포유증식유명현억제작용，대BSCC세포증식적무명현억제작용。재농도소우40μmol/L、작용시간12～24 h적정황하，CA대EJ화T24세포적증식억제작용구유명현적시간-효응화제량-효응관계，24 h시약물반수억제농도（IC50）위20μmol/L；재농도소우60μmol/L、작용시간24～48 h정황하，CA대BSCC적억제작용구유명현적시간-효응화제량-효응관계，48 h시약물IC50위60μmol/L。재농도소우80μmol/L、작용시간24～48 h정황하，NCTD대EJ화T24세포적증식억제작용구유명현적시간-효응화제량-효응관계；24 h시약물IC50위60μmol/L。격광공취초소묘현미경급전자현미경관찰증실CA화NCTD통과유도방광종류세포조망진이억제기증식。결론 CA급NCTD균구유교호적억제상견방광종세포증식적작용，유망용우천표방광종류전절술후적방광관주화료；차CA대BSCC세포증식구유교호적억제작용，유망용우방광종류전절표본중반발 BSCC환자적방광관주화료。
ObjectiveCantharidin (CA) is recently used as anti-tumor natural medicine in many cancers including bladder carcinoma, but the mechanisms are poorly understood. This study is aimed to explore the inhibit effects of CA and its derivative-norcantharidin (NCTD) on T24, EJ and squamous carcinoma cell (BSCC) of bladderin vitro.Methods Cultured carcinoma cells were treated with CA or NCTD at different concentrations for 12 h, 24 h, 36 h, 48 h and 60 h, then cell viability was measured by MTT assay. After treated by CA and NCTD at optimized concentrations, carcinoma cells cellular morphological changes were also investigated by using Laser Scanning Confocal Microscope (LSCM) and Electron Microscope.Results Human bladder cancer cell proliferation of EJ, T24 and BSCC cells significantly was inhibitedin vitroby CA. EJ and T24 cell proliferation obviously was inhibited by NCTD, and BSCC cell can not be inhibited by NCTD. In a concentration of less than 40 μmol/L, between 12-24 h, the inhibitory effect of CA on proliferation of EJ and T24 cells showed time-effect and dose-effect relationship, drug half inhibitory concentration (IC50) of 24 h is 20 μmol/L. In a concentration of less than 60 μmol/L, between 24-48 h, the inhibition effect of CA on BSCC showed time-effect and dose-effect obvious relationship, IC50 of 48 h was 0 μmol/L. In a concentration of less than 80 μmol/L, between 24-48 h,inhibitory effect of NCTD on proliferation of EJ and T24 cells showed time-effect and dose-effect relationship; IC50 of 24 h was 60 μmol/L. LSCM and electron microscopy confirmed that CA and NCTD inhibited the proliferation of bladder cancer cells through the inducting apoptosis. Both CA and NCTD were able to decrease BSCC viability in time dependent and dose dependent manners; the maximum effect dose was 60 μmol/L. When cells were treated with CA or NCTD at 60 μmol/L for 24 h or 36 h, apoptosis was induced in BSCC, more Apoptotic bodies were founded in CA group.ConclusionCA and NCTD can inhibit the proliferation of common bladder carcinoma cells, is expected to be used in bladder irrigation chemotherapy of superficial bladder tumor after transurethral resection. CA can inhibit the proliferation of BSCC cells, is expected to be used in bladder cancer electrocision specimens with bladder perfusion chemotherapy in patients with BSCC.