CAJ | 학술논문

目的：观察植物药有效成分三七总皂苷( PNS)与雷公藤多苷( TG)配伍对胶原诱导关节炎( CIA)大鼠血管和心肌损伤的保护效应。方法 Wistar大鼠50只中选6只为对照组,其余皮内注射鸡II型胶原建立CIA模型,成模后随机分为模型组、雷公藤多苷组( TG)、TG+PNS(三七总皂苷)组,每组6只。对照组、模型组生理盐水灌胃, TG组雷公藤多苷灌胃,TG+PNS组三七总皂苷配伍雷公藤多苷灌胃,共42 d。治疗结束后,腹主动脉取血,采用蛋白芯片技术检测白介素( IL)-6、纤溶酶原激活物抑制剂( PAI-l)含量；采血后立即开胸,取胸主动脉及心脏,进行病理学观察。结果模型组及TG组、TG+PNS组造模第21天、给药4、6周后体重及体重增加值明显低于对照组( P <0．01),PAI-1、IL-6表达明显高于对照组(P<0．01)；给药第4、6周TG+PNS组体重增加值明显高于模型组(P<0．05, P<0．01),血清PAI-1、IL-6水平显著降低(P<0．01),且PNS+TG组优于TG组(P<0．05)。模型组胸主动脉内皮不完整,可见炎细胞浸润、脂质沉积及泡沫细胞形成,心肌可见广泛炎性病变；TG组可见部分单核细胞、淋巴细胞等聚集,部分心肌细胞肥大；PNS+TG组内皮完整,未见明显病理改变,心肌仅见零星炎性细胞。结论 CIA大鼠存在心血管损伤,TG联合PNS在治疗关节炎的同时可能具有心血管保护作用。
목적：관찰식물약유효성분삼칠총조감( PNS)여뢰공등다감( TG)배오대효원유도관절염( CIA)대서혈관화심기손상적보호효응。방법 Wistar대서50지중선6지위대조조,기여피내주사계II형효원건립CIA모형,성모후수궤분위모형조、뢰공등다감조( TG)、TG+PNS(삼칠총조감)조,매조6지。대조조、모형조생리염수관위, TG조뢰공등다감관위,TG+PNS조삼칠총조감배오뢰공등다감관위,공42 d。치료결속후,복주동맥취혈,채용단백심편기술검측백개소( IL)-6、섬용매원격활물억제제( PAI-l)함량；채혈후립즉개흉,취흉주동맥급심장,진행병이학관찰。결과모형조급TG조、TG+PNS조조모제21천、급약4、6주후체중급체중증가치명현저우대조조( P <0．01),PAI-1、IL-6표체명현고우대조조(P<0．01)；급약제4、6주TG+PNS조체중증가치명현고우모형조(P<0．05, P<0．01),혈청PAI-1、IL-6수평현저강저(P<0．01),차PNS+TG조우우TG조(P<0．05)。모형조흉주동맥내피불완정,가견염세포침윤、지질침적급포말세포형성,심기가견엄범염성병변；TG조가견부분단핵세포、림파세포등취집,부분심기세포비대；PNS+TG조내피완정,미견명현병리개변,심기부견령성염성세포。결론 CIA대서존재심혈관손상,TG연합PNS재치료관절염적동시가능구유심혈관보호작용。
Objective To observe the protective effect of Panax Notoginsenosidum (PNS) combined with Tripterygi-um Glycosides ( TG) on the blood vessel and myocardial damage in rats with collagen induced arthritis ( CIA) . Methods Six rats from 50 Wistar rats were selected as control group, and the other rats were used to establish CIA models by giving intrader-mal injections of Chicken Collagen type II and successful models were randomly divided into model group (n=6), TG group (n=6) and TG+PNS group (n=6). The control and model groups were lavaged with physiological saline, and TG group was lavaged with TG, while TG+PNS group was lavaged with TG+PNS for 42 d. After the treatment, the serum from abdominal aortas was obtained, and the contents of TNF-α(tumor necrosis factor), IL-6 (interleukin) and PAI-1 (plasminogen activator inhibitor) were detected using protein chip technology;thoracic cavity was opened immediately to get the thoracic aorta and cardiac tissue for pathological observation. Results On the 21st d after models establishment, and in the 4th and 6th weeks after administration, the increased values of body weight in model, TG and TG+PNS groups were significantly lower than that in control group (P<0. 01), while levels of PAI-1 and IL-6 were significantly higher to those in control group(P<0. 01);in the 4th and 6th weeks after administration, the increased values of body weight in TG+PNS group were significantly higher than those in model group (P<0. 05, P<0. 01), while levels of PAI-1 and IL-6 were significantly decreased (P<0. 01), and the changes in PNS+TG group were superior to those in TG group (P<0. 05). The endothelium of thoracic aorta in model group was not integrated, and it had inflammatory cellular infiltration, lipidoses and foam cell formation with wide myocardial inflam-matory lesions. Part of mononuclear and lymphocyte cell aggregation was found in TG group with part of myocardial cells hyper-trophy. The endothelium of thoracic aorta in PNS+TG group was integrated without pathological change, and sporadically in-flammatory cells were found. Conclusion Rats with CIA suffer cardiovascular injury, and PNS+TG treatment may have a protective effect on the cardiovascular injury in rats with CIA during the treatment of arthritis.