CAJ | 학술논문

目的：检测不同病理级别原发性肝细胞癌中N-myc下游调节基因2( NDRG2)和蛋白激酶B2( AKT2) mRNA及其磷酸化蛋白的表达水平。方法收集不同病理级别原发性肝细胞癌组织及癌旁正常肝组织标本各30例,检测标本中NDRG2、AKT2 mRNA及其磷酸化蛋白的表达情况,并对其间的相互关系进行分析。结果30例原发性肝细胞癌组织中NDRG2 mRNA表达低于瘤旁组织(P<0．05),AKT2 mRNA与瘤旁组织无明显差异(P>0．05),两者均与病理级别无关(P>0．05),且NDRG2与AKT2表达无相关性(P>0．05)。在高表达474位丝氨酸磷酸化的AKT2蛋白(P-AKT2-SER474)分子的标本中,两个NDRG2磷酸化分子多为低表达,相反,在低表达P-AKT2-SER474分子的标本中,两个NDRG2磷酸化分子多为高表达。结论在mRNA表达水平,还不能认为NDRG2与AKT2之间有相关性。在蛋白质水平,NDRG2的磷酸化不依赖于AKT2的磷酸化,且NDRG2与AKT2的磷酸化作用可能存在竞争关系。
목적：검측불동병리급별원발성간세포암중N-myc하유조절기인2( NDRG2)화단백격매B2( AKT2) mRNA급기린산화단백적표체수평。방법수집불동병리급별원발성간세포암조직급암방정상간조직표본각30례,검측표본중NDRG2、AKT2 mRNA급기린산화단백적표체정황,병대기간적상호관계진행분석。결과30례원발성간세포암조직중NDRG2 mRNA표체저우류방조직(P<0．05),AKT2 mRNA여류방조직무명현차이(P>0．05),량자균여병리급별무관(P>0．05),차NDRG2여AKT2표체무상관성(P>0．05)。재고표체474위사안산린산화적AKT2단백(P-AKT2-SER474)분자적표본중,량개NDRG2린산화분자다위저표체,상반,재저표체P-AKT2-SER474분자적표본중,량개NDRG2린산화분자다위고표체。결론재mRNA표체수평,환불능인위NDRG2여AKT2지간유상관성。재단백질수평,NDRG2적린산화불의뢰우AKT2적린산화,차NDRG2여AKT2적린산화작용가능존재경쟁관계。
Objective To investigate the expressions of N-myc downstream regulated gene 2 (NDRG2), protein kinase B2 ( AKT2 ) mRNA and their phosphorylated proteins in human hepatic carcinoma cells ( HHCC ) of different pathological grades. Methods The expressions of NDRG2, AKT2 mRNA and their phosphorylated proteins in 30 cases of HHCCs ( group A) and 30 cases of adjacent normal tissues ( group B) were detected, and the correlations were also analyzed. Results The NDRG2 mRNA expression in group A was significantly lower than that in group B (P<0. 05), but there was no significant difference in AKT2 expression between the two groups ( P >0. 05 ) , and expressions of NDRG2 mRNA and AKT2 were not correlated with pathological grades, furthermore there was no statistically significant correlation between NDRG2 mRNA and AKT2 mRNA expressions (P>0. 05). The expressions of two kinds of phospho-rylated NDRG2 proteins were low in the samples in which the phosphorylated AKT2 protein serine474 ( P-AKT2-SER474) was highly expressed, and the expressions of two kinds of phosphorylated NDRG2 proteins were high in the samples where the P-AKT2-SER474 was slightly expressed. Conclusion The mRNA expression can not show the corre-lation between NDRG2 and AKT2. The protein levels show that the NDRG2 phosphorylation does not depend on the AKT2 phosphorylation, and competitive mechanisms may exist in the two processes.