现代畜牧兽医
現代畜牧獸醫
현대축목수의
LIAONING JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY MEDICINE
2015年
1期
33-39
,共7页
李海利%朗利敏%徐引弟%焦文强%朱文豪%张立宪%张青娴%游一%候自花%杨素新%许峰%郑万录%王克领
李海利%朗利敏%徐引弟%焦文彊%硃文豪%張立憲%張青嫻%遊一%候自花%楊素新%許峰%鄭萬錄%王剋領
리해리%랑리민%서인제%초문강%주문호%장립헌%장청한%유일%후자화%양소신%허봉%정만록%왕극령
猪胸膜肺炎放线杆菌%分子鉴定%血清2型
豬胸膜肺炎放線桿菌%分子鑒定%血清2型
저흉막폐염방선간균%분자감정%혈청2형
Porcine contagious pleuropneumonia Actinobacillus pleuropneumoniae%Molecular iden-tification%Serotype 2
猪接触性传染性胸膜肺炎(porcine contagious pleuropneumonia,PCP)又称坏死性胸膜肺炎,是由胸膜肺炎放线杆菌引起的一种高度接触传染性、致死性呼吸道传染病。以急性出血性纤维素性肺炎和慢性纤维素性坏死性胸膜炎为主要特征。各种年龄、性别的猪对本病均易感,急性者死亡率高,慢性者常能耐过。猪传染性胸膜肺炎放线杆菌有15个血清型,不同血清型之间没有或仅有较弱的交叉保护作用,这给猪传染性胸膜肺炎放线杆菌的诊断防治和免疫预防带来了很大的困难。因此建立一种快速血清型分子鉴定方法尤为重要。为了快速、简便的建立猪传染性胸膜肺炎放线杆菌血清型分子鉴定的方法,本研究所从临床发病疑似病例猪肺脏和气管中分离到的放线杆菌,首先经过血清型鉴定,确定部分血清型为2型,为了确定血清型鉴定的准确性,在此基础上,采用分子鉴定的方法,对这些菌株进行鉴定,确定分离到的11株菌为猪胸膜肺炎放线杆菌血清2型。这为猪传染性胸膜肺炎放线杆菌血清型分子鉴定及防治提供了理论依据,为今后临床血清型定型提供了一种简便方法。
豬接觸性傳染性胸膜肺炎(porcine contagious pleuropneumonia,PCP)又稱壞死性胸膜肺炎,是由胸膜肺炎放線桿菌引起的一種高度接觸傳染性、緻死性呼吸道傳染病。以急性齣血性纖維素性肺炎和慢性纖維素性壞死性胸膜炎為主要特徵。各種年齡、性彆的豬對本病均易感,急性者死亡率高,慢性者常能耐過。豬傳染性胸膜肺炎放線桿菌有15箇血清型,不同血清型之間沒有或僅有較弱的交扠保護作用,這給豬傳染性胸膜肺炎放線桿菌的診斷防治和免疫預防帶來瞭很大的睏難。因此建立一種快速血清型分子鑒定方法尤為重要。為瞭快速、簡便的建立豬傳染性胸膜肺炎放線桿菌血清型分子鑒定的方法,本研究所從臨床髮病疑似病例豬肺髒和氣管中分離到的放線桿菌,首先經過血清型鑒定,確定部分血清型為2型,為瞭確定血清型鑒定的準確性,在此基礎上,採用分子鑒定的方法,對這些菌株進行鑒定,確定分離到的11株菌為豬胸膜肺炎放線桿菌血清2型。這為豬傳染性胸膜肺炎放線桿菌血清型分子鑒定及防治提供瞭理論依據,為今後臨床血清型定型提供瞭一種簡便方法。
저접촉성전염성흉막폐염(porcine contagious pleuropneumonia,PCP)우칭배사성흉막폐염,시유흉막폐염방선간균인기적일충고도접촉전염성、치사성호흡도전염병。이급성출혈성섬유소성폐염화만성섬유소성배사성흉막염위주요특정。각충년령、성별적저대본병균역감,급성자사망솔고,만성자상능내과。저전염성흉막폐염방선간균유15개혈청형,불동혈청형지간몰유혹부유교약적교차보호작용,저급저전염성흉막폐염방선간균적진단방치화면역예방대래료흔대적곤난。인차건립일충쾌속혈청형분자감정방법우위중요。위료쾌속、간편적건립저전염성흉막폐염방선간균혈청형분자감정적방법,본연구소종림상발병의사병례저폐장화기관중분리도적방선간균,수선경과혈청형감정,학정부분혈청형위2형,위료학정혈청형감정적준학성,재차기출상,채용분자감정적방법,대저사균주진행감정,학정분리도적11주균위저흉막폐염방선간균혈청2형。저위저전염성흉막폐염방선간균혈청형분자감정급방치제공료이론의거,위금후림상혈청형정형제공료일충간편방법。
Porcine contagious pleuropneumonia also known as necrotic pleuropneumonia, is a high-ly contagious and lethal respiratory infectious disease caused by Actinobacillus pleuropneumoniae. The main characteristic of this disease are acute hemorrhagic fibrinous pneumonia and chronic fi-brinous necrosis. Pigs at all ages and gender were susceptible to this disease and caused acute mortality. Actinobacillus pleuropneumoniae has 15 serotypes, there are no or weakly cross protec-tion between different serotypes, which brought great difficulties to control , diagnosis and im-mune prevention this disease. So it is particularly important to establish a rapid molecular identification method for serotype. In order to establish a rapid, simple method to identify Porcine Contagious pleuropneumoniae serotype , this research identified Actinobacillus pleuropneumoniae from swine lung and trachea with clinical of suspection , through serotype identification, determined type 2 serotype. In order to determine the accuraate serotype, the method of molecular identification was studied. 11 isolated strains were identified as serotype 2 Actinobacillus pleuropneumoniae. This provides a theoretical basis for molecular identification the infectious porcine Actinobacillus pleuropneumoniae serotype , and provides a simple method for the future clinical serum identification.