现代仪器与医疗
現代儀器與醫療
현대의기여의료
Modern Instrument and Medical Treatment
2015年
1期
1-4
,共4页
张力%段秉亚%秦功伟%仓怀兴
張力%段秉亞%秦功偉%倉懷興
장력%단병아%진공위%창부흥
蛋白质芯片%毛细管电泳%紫外检测%凝胶基板%蛋白质微阵列
蛋白質芯片%毛細管電泳%紫外檢測%凝膠基闆%蛋白質微陣列
단백질심편%모세관전영%자외검측%응효기판%단백질미진렬
Protein chip%Capillary electrophoresis%Ultraviolet detection%Gel-base%Protein microarray
设计构建了具有紫外实时检测功能的凝胶基蛋白质芯片制备系统，借助毛细管电泳技术制备出凝胶基蛋白质阵列。芯片制备系统包括高压电源、毛细管电泳、点样阵列化和紫外定量检测4个功能模块，其中高压电源具备可调输出功能，毛细管电泳加装了超声波振动除气泡机构，点样阵列化模块采用微米级三维位移台，紫外定量检测模块使用了280n m单色L E D。通过调节电泳电压、蛋白质浓度、毛细管直径以及点样停留时间等参数，可以实现对样点大小和蛋白质含量的调节。
設計構建瞭具有紫外實時檢測功能的凝膠基蛋白質芯片製備繫統，藉助毛細管電泳技術製備齣凝膠基蛋白質陣列。芯片製備繫統包括高壓電源、毛細管電泳、點樣陣列化和紫外定量檢測4箇功能模塊，其中高壓電源具備可調輸齣功能，毛細管電泳加裝瞭超聲波振動除氣泡機構，點樣陣列化模塊採用微米級三維位移檯，紫外定量檢測模塊使用瞭280n m單色L E D。通過調節電泳電壓、蛋白質濃度、毛細管直徑以及點樣停留時間等參數，可以實現對樣點大小和蛋白質含量的調節。
설계구건료구유자외실시검측공능적응효기단백질심편제비계통，차조모세관전영기술제비출응효기단백질진렬。심편제비계통포괄고압전원、모세관전영、점양진렬화화자외정량검측4개공능모괴，기중고압전원구비가조수출공능，모세관전영가장료초성파진동제기포궤구，점양진렬화모괴채용미미급삼유위이태，자외정량검측모괴사용료280n m단색L E D。통과조절전영전압、단백질농도、모세관직경이급점양정류시간등삼수，가이실현대양점대소화단백질함량적조절。
A system for building gel-based protein chip with the function of ultraviolet real-time detection was designed and constructed successfully. The gel-based protein microarray was fabricated using the system with the aid of capillary electrophoresis technology. The system includes four modules: power supply of high voltage, capillary electrophoresis, protein sample dotting, ultraviolet quantitative detection. The outlet voltage of the power supply of high voltage module could be adjusted. The capillary electrophoresis module is equipped with ultrasonic vibration device for removing micro bubbles. The protein sample dotting module adopts the three dimensional stage of mm precision. The ultraviolet quantitative detection module uses the LED of 280nm. The sample dot size and protein content could be modulated through changing electrophoresis voltage, protein concentration, capillary diameter and electrophoretic time.
