CAJ | 학술논문

目的：探讨转化生长因子α（ transforming growth factor α，TGF-α）对人内皮祖细胞（ endothelial pro-genitor cells，EPCs）单克隆形成、增殖、迁移和黏附细胞功能的影响及机制。方法：分离培养人内皮祖细胞，分别用浓度为1、5、10μg／L TGF-α诱导处理细胞，并设置PBS对照组和表皮生长因子受体酪氨酸激酶抑制剂EGFR-TKI组（同时加入10μg／L TGF-α和1∶1000的EGFR-TKI）。利用单克隆形成实验、MTT法和EdU法、Transwell法和细胞黏附实验检测不同浓度TGF-α对各组EPCs单克隆形成能力、细胞增殖、细胞迁移能力及黏附功能的影响；并通过Western blotting法检测不同浓度TGF-α对各组EPCs中表皮生长因子受体（ epithelial growth factor receptor ，EG-FR）和血管内皮生长因子（vascular endothelial growth factor ，VEGF）表达的影响。结果：不同浓度TGF-α均能显著诱导EPCs单克隆形成能力、增殖、迁移和黏附细胞功能，差异有统计学意义（P＜0.05），并可以被EGFR-TKI抑制。Western blotting检测发现TGF-α显著诱导EPCs中EGFR和VEGF的表达（ P＜0.05），并呈浓度依赖性。结论：TGF-α能够显著促进人EPCs细胞单克隆形成、增殖、迁移和黏附相关细胞功能，并通过与EGFR结合诱导VEGF表达来发挥作用。
목적：탐토전화생장인자α（ transforming growth factor α，TGF-α）대인내피조세포（ endothelial pro-genitor cells，EPCs）단극륭형성、증식、천이화점부세포공능적영향급궤제。방법：분리배양인내피조세포，분별용농도위1、5、10μg／L TGF-α유도처리세포，병설치PBS대조조화표피생장인자수체락안산격매억제제EGFR-TKI조（동시가입10μg／L TGF-α화1∶1000적EGFR-TKI）。이용단극륭형성실험、MTT법화EdU법、Transwell법화세포점부실험검측불동농도TGF-α대각조EPCs단극륭형성능력、세포증식、세포천이능력급점부공능적영향；병통과Western blotting법검측불동농도TGF-α대각조EPCs중표피생장인자수체（ epithelial growth factor receptor ，EG-FR）화혈관내피생장인자（vascular endothelial growth factor ，VEGF）표체적영향。결과：불동농도TGF-α균능현저유도EPCs단극륭형성능력、증식、천이화점부세포공능，차이유통계학의의（P＜0.05），병가이피EGFR-TKI억제。Western blotting검측발현TGF-α현저유도EPCs중EGFR화VEGF적표체（ P＜0.05），병정농도의뢰성。결론：TGF-α능구현저촉진인EPCs세포단극륭형성、증식、천이화점부상관세포공능，병통과여EGFR결합유도VEGF표체래발휘작용。
AIM:To explore the effects of transforming growth factor-α( TGF-α) in the monoclonal formation , proliferation, migration and adhesiveness of human endothelial progenitor cells ( EPCs).METHODS: The isolated and cultured EPCs were treated with various concentrations of TGF-α(final concentrations of 1, 5, 10μg/L, respectively).At the same time, the PBS control and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) group (10μg/L TGF-αplus 1∶1 000 EGFR-TKI) were set.The effects of TGF-αon monoclonal formation , proliferation, migration and adhesiveness of EPCs were determined by clone formation experiment , thiazolyl blue tetrazolium bromide (MTT), EdU, Transwell and adhesion assays , respectively.The expression of epithelial growth receptor (EGFR) and vascular endothelial growth factor ( VEGF) were measured by Western blotting .RESULTS:Different concentrations of TGF-αall significantly induced the monoclonal formation , proliferation, migration and adhesiveness of EPCs (P<0.01), which were inhibited by EGFR-TKI.The results of Western blotting showed that TGF-αalso induced the expression of EGFR and VEGF with a cer-tain concentration effect ( P<0.01) .CONCLUSION:By combining with EGFR induced the expression of VEGF , TGF-αsignificantly promotes the related cell function of monoclonal formation , proliferation, migration, adhesiveness in EPCs.