CAJ | 학술논문

目的：明确飞机草镇痛作用部位及其可能的作用机制，为充分合理利用飞机草资源提供参考。方法将小鼠分成6组，采用脊髓化小鼠甩尾法，福尔马林致炎性疼痛法及纳洛酮阻断小鼠扭体法、热板法，分析飞机草镇痛作用部位；应用酶联免疫法检测血清和脑组织中NO和前列腺素E2（PGE2）含量，初步研究其镇痛作用机制。结果实验结果表明高剂量的飞机草能明显提高脊髓化小鼠热刺激甩尾反应痛阈值（P＜0．05）；高、中剂量的飞机草对福尔马林致痛早期相、晚期相均有显著镇痛作用（P＜0．05），明显减少纳洛酮阻断后冰醋酸性腹膜炎症致痛小鼠扭体次数，并明显延长纳洛酮阻断后热板致痛小鼠舔后足时间（P＜0．05），能显著降低福尔马林致炎疼痛小鼠脑组织和血清中NO和PGE2含量（P＜0．05）。结论飞机草既有中枢也有外周的镇痛作用，这可能与其降低中枢和外周NO和PGE2含量有关。
목적：명학비궤초진통작용부위급기가능적작용궤제，위충분합리이용비궤초자원제공삼고。방법장소서분성6조，채용척수화소서솔미법，복이마림치염성동통법급납락동조단소서뉴체법、열판법，분석비궤초진통작용부위；응용매련면역법검측혈청화뇌조직중NO화전렬선소E2（PGE2）함량，초보연구기진통작용궤제。결과실험결과표명고제량적비궤초능명현제고척수화소서열자격솔미반응통역치（P＜0．05）；고、중제량적비궤초대복이마림치통조기상、만기상균유현저진통작용（P＜0．05），명현감소납락동조단후빙작산성복막염증치통소서뉴체차수，병명현연장납락동조단후열판치통소서첨후족시간（P＜0．05），능현저강저복이마림치염동통소서뇌조직화혈청중NO화PGE2함량（P＜0．05）。결론비궤초기유중추야유외주적진통작용，저가능여기강저중추화외주NO화PGE2함량유관。
Objective To study the analgesic effect of Eupatorium odoratum L and its mechanism .Methods Mice were random‐ly divided into 6 groups .Swinging tail method in spinal cord destroyed mouse ,hot‐plate and twisted body method in naloxone blocked mouse ,and formalin‐induce inflammatory pain method were done to investigate the analgesic effect of Eupatorium odoratum L .Each pain index under each experiment condition was determined after intra gastric administration of each group of mice .The concentration of NO and PGE2 in serum and brain were measured to study its mechanisms .Results High‐dose of Eupatorium odor‐atum L significantly enhanced pain threshold in either spinal cord destroyed or naloxone blocked mouse with pain induced by hot and acetic acid(P<0 .05) .High and middle dosage groups of Eupatorium odoratum L markedly reduced the first‐and second‐phase re‐sponses of formalin‐induced inflammatory pain mouse(P< 0 .05) ,and decreased the writhing times and licking rear feet times caused by twisted body method(P<0 .05) .The level of NO and PGE2 in serum and brain of mouse induced by formalin were both decreased(P<0 .05) .Conclusion Eupatorium odoratum L has notable analgesic effect ,which may be related to reduce the level of NO and PGE2 in central and peripheral site .