重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
3期
306-308,311
,共4页
肝内胆汁淤积%大黄素%c-kit%胆管ICC
肝內膽汁淤積%大黃素%c-kit%膽管ICC
간내담즙어적%대황소%c-kit%담관ICC
intrahepatic cholestasis%emodin%c-kit%interstitial cells of Cajal in bile duct
目的：通过研究大黄素对药物性肝内胆汁淤积症模型相关的生化指标及Cajal间质细胞（ICC）的影响，以期发现胆管ICC在肝内胆汁淤积中的作用及大黄素对ICC及肝内胆汁淤积的影响。方法15只雄性SD大鼠采用简单随机法均分为3组，即对照组、药物性肝内胆汁淤积模型组及大黄素干预组（n＝5）。建立大鼠淤胆型肝炎及大黄素干预的模型。使用反转录PCR（RT‐PCR）及免疫组织化学法分别检测模型组、大黄素组及对照组间肝功能生化指标、干细胞因子受体c‐kit基因及蛋白表达水平。结果模型组肝功能生化指标水平与对照组相比明显升高（P＜0．05），大黄素组上述指标水平较对照组也有明显升高，但较模型组低，其间差异有统计学意义（P＜0．05）。对照组、模型组及大黄素组位于548bp处的条带均有表达。模型组c‐kitmR‐NA表达量明显低于干预组及对照组（P＜0．05）。大黄素组低于对照组，组间差异无统计学意义（P＞0．05）。免疫组织化学结果显示c‐kit模型组中表达量明显低于对照组及大黄素组（P＜0．05）。结论胆管ICC与药物性肝内胆汁淤积形成过程可能存在密切关系，肝内胆汁淤积的形成与胆管ICC减少有关，而大黄素对肝内胆汁淤积的治疗作用可能与其所致的胆管ICC数量相对增多或对胆管ICC的保护作用有关。
目的：通過研究大黃素對藥物性肝內膽汁淤積癥模型相關的生化指標及Cajal間質細胞（ICC）的影響，以期髮現膽管ICC在肝內膽汁淤積中的作用及大黃素對ICC及肝內膽汁淤積的影響。方法15隻雄性SD大鼠採用簡單隨機法均分為3組，即對照組、藥物性肝內膽汁淤積模型組及大黃素榦預組（n＝5）。建立大鼠淤膽型肝炎及大黃素榦預的模型。使用反轉錄PCR（RT‐PCR）及免疫組織化學法分彆檢測模型組、大黃素組及對照組間肝功能生化指標、榦細胞因子受體c‐kit基因及蛋白錶達水平。結果模型組肝功能生化指標水平與對照組相比明顯升高（P＜0．05），大黃素組上述指標水平較對照組也有明顯升高，但較模型組低，其間差異有統計學意義（P＜0．05）。對照組、模型組及大黃素組位于548bp處的條帶均有錶達。模型組c‐kitmR‐NA錶達量明顯低于榦預組及對照組（P＜0．05）。大黃素組低于對照組，組間差異無統計學意義（P＞0．05）。免疫組織化學結果顯示c‐kit模型組中錶達量明顯低于對照組及大黃素組（P＜0．05）。結論膽管ICC與藥物性肝內膽汁淤積形成過程可能存在密切關繫，肝內膽汁淤積的形成與膽管ICC減少有關，而大黃素對肝內膽汁淤積的治療作用可能與其所緻的膽管ICC數量相對增多或對膽管ICC的保護作用有關。
목적：통과연구대황소대약물성간내담즙어적증모형상관적생화지표급Cajal간질세포（ICC）적영향，이기발현담관ICC재간내담즙어적중적작용급대황소대ICC급간내담즙어적적영향。방법15지웅성SD대서채용간단수궤법균분위3조，즉대조조、약물성간내담즙어적모형조급대황소간예조（n＝5）。건립대서어담형간염급대황소간예적모형。사용반전록PCR（RT‐PCR）급면역조직화학법분별검측모형조、대황소조급대조조간간공능생화지표、간세포인자수체c‐kit기인급단백표체수평。결과모형조간공능생화지표수평여대조조상비명현승고（P＜0．05），대황소조상술지표수평교대조조야유명현승고，단교모형조저，기간차이유통계학의의（P＜0．05）。대조조、모형조급대황소조위우548bp처적조대균유표체。모형조c‐kitmR‐NA표체량명현저우간예조급대조조（P＜0．05）。대황소조저우대조조，조간차이무통계학의의（P＞0．05）。면역조직화학결과현시c‐kit모형조중표체량명현저우대조조급대황소조（P＜0．05）。결론담관ICC여약물성간내담즙어적형성과정가능존재밀절관계，간내담즙어적적형성여담관ICC감소유관，이대황소대간내담즙어적적치료작용가능여기소치적담관ICC수량상대증다혹대담관ICC적보호작용유관。
Objective To study the effect of emodin on biochemical indicators of drug‐induced intrahepatic cholestasis model and the interstitial cells of cajal (ICC) in bile duct and to explore the role of ICC and emodin in intrahepatic cholestasis .Methods Fif‐teen rats were randomly divided into drug‐induced intrahepatic cholestasis group ,emodin intervention group and control group(n=5) .Rat cholestatic hepatitis model and emodin intervention model were established .RT‐PCR and immunohistochemistry were used to detect liver function ,c‐kit mRNA and protein expression levels in drug‐induced intrahepatic cholestasis group ,emodin interven‐tion group and control group .Results The degree of liver dysfunction and bilirubin level in drug‐induced intrahepatic cholestasis group were significantly higher than those in control group(P<0 .05);the above indicators in emodin intervention group were sig‐nificantly higher than those in control group but lower than those in drug‐induced intrahepatic cholestasis group(P<0 .05) .The c‐kit mRNA expression located at 548 bp was observed in control group ,emodin intervention group and drug‐induced intrahepatic cholestasis group .Relative expression level of c‐kit mRNA in drug‐induced intrahepatic cholestasis group was significantly lower than that in emodin intervention group and control group (P<0 .05) .Meanwhile ,there was no significant difference in relative ex‐pression level of c‐kit mRNA between emodin intervention group and control group (P>0 .05) .Immunohistochemistry results indi‐cated that expression of c‐kit in drug‐induced intrahepatic cholestasis group was significantly lower than those in control group and emodin intervention group(P<0 .05) .Conclusion There may be close relationship between the forming process of drug‐induced in‐trahepatic cholestasis and decrease of ICC in bile duct .The therapeutic effect of emodin on intrahepatic cholestasis may be related with the number of ICC in bile duct or the positive effect on ICC.
