CAJ | 학술논문

目的：研究幽门螺旋杆菌（简称幽门螺杆菌）感染小鼠胃黏膜组织细胞因子变化，初步探讨幽门螺杆菌感染的免疫机制。方法选取8～10周龄BALB／c小鼠24只，雌雄各半，分为观察组和对照组，两组造模前均禁食12h，观察组以2×109CFU／mL浓度的幽门螺杆菌菌液0．5mL／d灌胃，连续7d。对照组灌胃给生理盐水，0．5mL／d，连续7d，进行幽门螺杆菌感染小鼠胃黏膜造模。采用实时定量荧光PCR（Real‐timePCR）检测检测感染2、4周后，小鼠血浆中γ干扰素（IFN‐γ）、重组改构人肿瘤坏死因子α（TNF‐α）、白细胞介素‐8（IL‐8）以及IL‐10mRNA的表达量，并于感染4周后，处死小鼠，无菌取各组小鼠的胃组织，采用酶联免疫吸附试验夹心法检测胃黏膜组织中IFN‐γ、TNF‐α、IL‐8及IL‐10蛋白的水平。结果两组小鼠感染2周后，观察组IL‐8高于对照组，差异有统计学意义（P＜0．05）。感染4周后，IFN‐γ、TNF‐α、IL‐8的mRNA及蛋白表达量均明显上升，但观察组上升幅度明显高于对照组，差异有统计学意义（P＜0．05）。结论TH2细胞在幽门螺杆菌感染中的作用表现不明显，能促进TH1型细胞细胞因子的表达，引发TH1为主的免疫反应。
목적：연구유문라선간균（간칭유문라간균）감염소서위점막조직세포인자변화，초보탐토유문라간균감염적면역궤제。방법선취8～10주령BALB／c소서24지，자웅각반，분위관찰조화대조조，량조조모전균금식12h，관찰조이2×109CFU／mL농도적유문라간균균액0．5mL／d관위，련속7d。대조조관위급생리염수，0．5mL／d，련속7d，진행유문라간균감염소서위점막조모。채용실시정량형광PCR（Real‐timePCR）검측검측감염2、4주후，소서혈장중γ간우소（IFN‐γ）、중조개구인종류배사인자α（TNF‐α）、백세포개소‐8（IL‐8）이급IL‐10mRNA적표체량，병우감염4주후，처사소서，무균취각조소서적위조직，채용매련면역흡부시험협심법검측위점막조직중IFN‐γ、TNF‐α、IL‐8급IL‐10단백적수평。결과량조소서감염2주후，관찰조IL‐8고우대조조，차이유통계학의의（P＜0．05）。감염4주후，IFN‐γ、TNF‐α、IL‐8적mRNA급단백표체량균명현상승，단관찰조상승폭도명현고우대조조，차이유통계학의의（P＜0．05）。결론TH2세포재유문라간균감염중적작용표현불명현，능촉진TH1형세포세포인자적표체，인발TH1위주적면역반응。
Objective To study the change of cytokines in gastric mucosa of mice with Helicobacter pylori infection and explore the immune mechanism .Methods Twenty‐four 8-10 weeks‐old BALB/c mice were randomly divided into observation group and control group .After first 12 hours of fasting ,observation group was given 0 .5 mL/d Helicobacter pylori bacterial liquid with con‐centration of 2 × 109 CFU/mL for 7 consecutive days .The control group was fed with 0 .5 mL/d saline for 7 consecutive days .Then the mice were infected with Helicobacter pylori gast model was established .Using Real‐time PCR to detect the expressions of IFN‐γ ,TNF‐α,IL‐8 and IL‐10 in murine plasma within 2 ,4 weeks after infection .And after 4 weeks of infection ,mice were sacrificed , and their stomach tissue sterile were separated .ELISA was used to detect the levels of IFN‐γ,TNF‐α,IL‐8 ,and IL‐10 mRNA in gastric mucosa .Results There was significantly difference between groups 2 weeks after infection(P<0 .05) .With lastingness of infection ,the expression and the mRNA of IFN‐γ,TNF‐α,IL‐8 were significantly increased ,and the rate of increase of observation group was higher(P<0 .05) .Conclusion TH2 cells may have no effect on Helicobacter pylori infection in mice gastric mucosa and promote the expression of T H1‐type cytokines ,triggering immune response of T H1 .