CAJ | 학술논문

目的：观察兔玻璃体腔注射鼠神经生长因子（mNGF）的安全性及其在视网膜组织的分布。方法兔玻璃体腔注射临床剂型mNGF30μg／100μL后1、7、30d进行行为学观察、裂隙灯眼前节检查、眼底检查、眼部B超及组织病理学检查，确定其使用安全性。玻璃体腔注射该剂量标记后的125I‐NGF15、30min，1、3、6、8、12、24、48h，观察视网膜药物峰浓度及达峰时间。结果兔眼玻璃体腔注射mNGF后，眼前节及眼底均未见明显损伤及炎性反应，眼部B超及组织HE染色未见明显眼组织结构异常。视网膜药物含量可于注射后3h达峰值（118．32±18．74）％ID／g，为眼部各组织中最高。结论玻璃体腔注射mNGF是安全的，玻璃体腔注射mNGF能够迅速在视网膜组织中聚集，达到较高药物浓度。
목적：관찰토파리체강주사서신경생장인자（mNGF）적안전성급기재시망막조직적분포。방법토파리체강주사림상제형mNGF30μg／100μL후1、7、30d진행행위학관찰、렬극등안전절검사、안저검사、안부B초급조직병이학검사，학정기사용안전성。파리체강주사해제량표기후적125I‐NGF15、30min，1、3、6、8、12、24、48h，관찰시망막약물봉농도급체봉시간。결과토안파리체강주사mNGF후，안전절급안저균미견명현손상급염성반응，안부B초급조직HE염색미견명현안조직결구이상。시망막약물함량가우주사후3h체봉치（118．32±18．74）％ID／g，위안부각조직중최고。결론파리체강주사mNGF시안전적，파리체강주사mNGF능구신속재시망막조직중취집，체도교고약물농도。
Objective To observe safety of intravitreal injection of mouse nerve growth factor and its distribution in retina in rabbits .Methods The behavioral observation ,slit lamp examination ,fundus examination ,eye B ultrasonic and histopathological ex‐amination were carried out on 1 ,7 and 30 d after intravitreal injection 30 μg/100 μL mNGF to determine the safety in eye .The dis‐tribution and peak time in retina were investigated at 15 ,30 min ,1 ,3 ,6 ,8 ,12 ,24 ,48 h after intravitreal injection 125 I‐NGF 30 μg/100 μL .Results No abnormal changes were found in their cornea ,lens ,vitreous body and retina after mNGF intravitreal injection . And the each layer of retinal cells layout were regular according to the result of morphological observation on 30 days after treat‐ment .The peak concentration of mNGF in retina and the highest in whole eye was (118 .32 ± 18 .74)% ID/g and the peak time was at 3 hour after injection .Conclusion It is safe for intravitreal injection of mNGF and mNGF could gather in retina quickly after in‐travitreal injection .