中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
1期
54-58
,共5页
卜晨峰%庾淦深%徐银%苏志坚%黄亚东%苏泽轩
蔔晨峰%庾淦深%徐銀%囌誌堅%黃亞東%囌澤軒
복신봉%유감심%서은%소지견%황아동%소택헌
1 ,2-二甲磺酸乙烷%肾脏%肾损伤分子1%睾酮
1 ,2-二甲磺痠乙烷%腎髒%腎損傷分子1%睪酮
1 ,2-이갑광산을완%신장%신손상분자1%고동
Ethane 1,2-dimethanesulfonate%Kidney%Kidney injury molecule-1%Testosterone
目的：探究1，2-二甲磺酸乙烷（ EDS）预处理是否对雄性SD大鼠缺血再灌注肾脏损伤有保护作用及其可能机制。方法：夹闭双侧肾蒂45 min，构建雄性SD大鼠肾脏缺血再灌注模型；48只雄性SD大鼠随机分为6组：空白对照组（blank）、假手术组（sham）、肾脏缺血再灌注组（I／R）、EDS预处理＋肾缺血再灌注组（EDS＋I／R）、EDS预处理＋睾酮＋肾缺血再灌注组（ EDS＋TST＋I／R）和阉割＋肾缺血再灌注组（ Cast＋I／R）。术前1 h及再灌注后24 h采血检测血清黄体生成素、睾酮、血肌酐、血尿素氮以及尿液肾损伤分子1（ KIM-1）水平；取肾脏组织，检测TNF-α水平、Fas mRNA与caspase-3蛋白表达情况。结果：（1） EDS＋I／R与Cast＋I／R组比较，血肌酐和尿素氮无显著差异（P＞0.05），但KIM-1水平却低于Cast＋I／R组（P＜0.05），2组血肌酐、血尿素氮和KIM-1均高于另外3组（P＜0.05）。（2）与术前1 h相比，再灌注术后24 h，EDS＋I／R、Cast＋I／R及EDS＋TST＋I／R组均伴睾酮及黄体生成素下降（P＜0.05）。（3） EDS＋I／R组TNF-α水平、Fas mRNA及caspase-3蛋白表达水平均显著低于Cast＋I／R与I／R组（ P＜0.05）。结论：EDS预处理可大幅降低血清睾酮水平，从而减轻雄性SD大鼠肾脏缺血再灌注损伤，其机制可能与TNF-α介导的Fas／FasL通路有关。
目的：探究1，2-二甲磺痠乙烷（ EDS）預處理是否對雄性SD大鼠缺血再灌註腎髒損傷有保護作用及其可能機製。方法：夾閉雙側腎蒂45 min，構建雄性SD大鼠腎髒缺血再灌註模型；48隻雄性SD大鼠隨機分為6組：空白對照組（blank）、假手術組（sham）、腎髒缺血再灌註組（I／R）、EDS預處理＋腎缺血再灌註組（EDS＋I／R）、EDS預處理＋睪酮＋腎缺血再灌註組（ EDS＋TST＋I／R）和閹割＋腎缺血再灌註組（ Cast＋I／R）。術前1 h及再灌註後24 h採血檢測血清黃體生成素、睪酮、血肌酐、血尿素氮以及尿液腎損傷分子1（ KIM-1）水平；取腎髒組織，檢測TNF-α水平、Fas mRNA與caspase-3蛋白錶達情況。結果：（1） EDS＋I／R與Cast＋I／R組比較，血肌酐和尿素氮無顯著差異（P＞0.05），但KIM-1水平卻低于Cast＋I／R組（P＜0.05），2組血肌酐、血尿素氮和KIM-1均高于另外3組（P＜0.05）。（2）與術前1 h相比，再灌註術後24 h，EDS＋I／R、Cast＋I／R及EDS＋TST＋I／R組均伴睪酮及黃體生成素下降（P＜0.05）。（3） EDS＋I／R組TNF-α水平、Fas mRNA及caspase-3蛋白錶達水平均顯著低于Cast＋I／R與I／R組（ P＜0.05）。結論：EDS預處理可大幅降低血清睪酮水平，從而減輕雄性SD大鼠腎髒缺血再灌註損傷，其機製可能與TNF-α介導的Fas／FasL通路有關。
목적：탐구1，2-이갑광산을완（ EDS）예처리시부대웅성SD대서결혈재관주신장손상유보호작용급기가능궤제。방법：협폐쌍측신체45 min，구건웅성SD대서신장결혈재관주모형；48지웅성SD대서수궤분위6조：공백대조조（blank）、가수술조（sham）、신장결혈재관주조（I／R）、EDS예처리＋신결혈재관주조（EDS＋I／R）、EDS예처리＋고동＋신결혈재관주조（ EDS＋TST＋I／R）화엄할＋신결혈재관주조（ Cast＋I／R）。술전1 h급재관주후24 h채혈검측혈청황체생성소、고동、혈기항、혈뇨소담이급뇨액신손상분자1（ KIM-1）수평；취신장조직，검측TNF-α수평、Fas mRNA여caspase-3단백표체정황。결과：（1） EDS＋I／R여Cast＋I／R조비교，혈기항화뇨소담무현저차이（P＞0.05），단KIM-1수평각저우Cast＋I／R조（P＜0.05），2조혈기항、혈뇨소담화KIM-1균고우령외3조（P＜0.05）。（2）여술전1 h상비，재관주술후24 h，EDS＋I／R、Cast＋I／R급EDS＋TST＋I／R조균반고동급황체생성소하강（P＜0.05）。（3） EDS＋I／R조TNF-α수평、Fas mRNA급caspase-3단백표체수평균현저저우Cast＋I／R여I／R조（ P＜0.05）。결론：EDS예처리가대폭강저혈청고동수평，종이감경웅성SD대서신장결혈재관주손상，기궤제가능여TNF-α개도적Fas／FasL통로유관。
AIM:To investigate the effects of ethane 1,2-dimethanesulfonate (EDS) preconditioning on renal ischemia/reperfusion (I/R) injury in male Sprague-Dawley (SD) rats.METHODS: Male SD rats (n=48) were ran-domly assigned to 6 groups:blank, sham, I/R, EDS+I/R, EDS+testosterone (TST) +I/R, and castration (Cast)+I/R.The renal pedicles were bilaterally occluded with a microvascular clamp for 45 min to establish renal I/R-induced in-jury model.Bilateral orchiectomy was conducted 2 weeks before surgery .EDS (75 mg/kg) was intraperitoneally injected 5 d before operation .Blood samples were collected 24 h after reperfusion from the vena orbitalis posterior plexus .Luteinizing hormone (LH), TST, serum creatinine (SCr), blood urea nitrogen (BUN), and kidney injury molecule-1 (KIM-1) were detected.The renal tissues were harvested to measure the level of TNF-αand the expression of Fas mRNA and caspase-3 protein.RESULTS:Serum TST levels in EDS +I/R group and Cast +I/R group were below the minimum detectable threshold.Compared with other groups , the rats in EDS+I/R group and Cast +I/R group had higher levels of SCr , BUN and KIM-1 (P<0.05).SCr and BUN levels showed no significant difference between EDS +I/R group and Cast +I/R group (P>0.05), but KIM-1 level in EDS+I/R group was lower than that in Cast +I/R group (P<0.05).After reper-fusion for 24 h, the levels of TST and LH in EDS +I/R group, Cast+I/R group and EDS+TST+I/R group were lower than those 1 h before operation (P<0.05).Compared with Cast+I/R and I/R group, the TNF-αlevel and expression of Fas mRNA and caspase-3 protein were significantly decreased in EDS +I/R group ( P<0.05 ) .CONCLUSION: EDS preconditioning substantially reduces the serum TST level , thus attenuating I/R-induced acute renal injury .TNF-α-induced Fas/FasL pathway may be involved in this process .
