CAJ | 학술논문

目的：研究亚硒酸钠（ Na2 SeO3）对中波紫外线（ UVB）损伤人角质形成细胞的保护作用。方法：培养永生化人角质形成细胞（HaCaT细胞），实验分为4组处理：（1）正常对照组；（2）Na2SeO3组：分别加入1 nmol／L、10 nmol／L、50 nmol／L、100 nmol／L、200 nmol／L和1μmol／L的Na2 SeO3预孵育24 h；（3） UVB组：300、600和900 J／m2 UVB照射；（4）Na2SeO3＋UVB组：Na2SeO3预孵育24 h后进行UVB照射。采用MTT法检测细胞增殖活性，采用流式细胞仪检测300 J／m2 UVB照射后细胞的凋亡率。结果：（1） UVB组与正常对照组比较，细胞增殖活性显著降低（P＜0.05），细胞活性与UVB照射剂量呈负相关；（2）Na2SeO3组与正常对照组比较，细胞增殖活性无明显差异；（3）不同浓度Na2SeO3＋UVB组与UVB组比较，细胞增殖活性增加，差异显著（P＜0.05），其中100 nmol／L组促进细胞增殖作用最强；（4）300 J／m2 UVB照射后，不同浓度Na2 SeO3＋UVB组与UVB组比较，凋亡率下降，差异显著（ P＜0.05），其中100 nmol／L组抑制凋亡作用最强。结论： UVB对角质形成细胞有损伤作用，且与照射剂量呈正相关；Na2 SeO3具有光保护性能，可减轻UVB辐射损伤人角质形成细胞。
목적：연구아서산납（ Na2 SeO3）대중파자외선（ UVB）손상인각질형성세포적보호작용。방법：배양영생화인각질형성세포（HaCaT세포），실험분위4조처리：（1）정상대조조；（2）Na2SeO3조：분별가입1 nmol／L、10 nmol／L、50 nmol／L、100 nmol／L、200 nmol／L화1μmol／L적Na2 SeO3예부육24 h；（3） UVB조：300、600화900 J／m2 UVB조사；（4）Na2SeO3＋UVB조：Na2SeO3예부육24 h후진행UVB조사。채용MTT법검측세포증식활성，채용류식세포의검측300 J／m2 UVB조사후세포적조망솔。결과：（1） UVB조여정상대조조비교，세포증식활성현저강저（P＜0.05），세포활성여UVB조사제량정부상관；（2）Na2SeO3조여정상대조조비교，세포증식활성무명현차이；（3）불동농도Na2SeO3＋UVB조여UVB조비교，세포증식활성증가，차이현저（P＜0.05），기중100 nmol／L조촉진세포증식작용최강；（4）300 J／m2 UVB조사후，불동농도Na2 SeO3＋UVB조여UVB조비교，조망솔하강，차이현저（ P＜0.05），기중100 nmol／L조억제조망작용최강。결론： UVB대각질형성세포유손상작용，차여조사제량정정상관；Na2 SeO3구유광보호성능，가감경UVB복사손상인각질형성세포。
AIM: To investigate the protective effect of sodium selenite ( Na2 SeO3 ) on human keratinocytes under ultraviolet-B (UVB) irradiation.METHODS: The cultured HaCaT cells were divided into 4 groups: (1) normal control group;(2) Na2 SeO3 group:pretreated with Na2 SeO3 at doses of 10 nmol/L, 50 nmol/L, 100 nmol/L, 200 nmol/L and 1 μmol/L for 24 h;(3) UVB group: irradiated with UVB at doses of 300, 600 and 900 J/m2; (4) Na2SeO3 +UVB group:after pretreated with Na2SeO3 for 24 h, irradiated with UVB at doses of 300, 600 and 900 J/m2.The cell pro-liferation was detected by MTT assay .The apoptotic rates of HaCaT cells treated with UVB at dose of 300 J/m2 were as-sessed by flow cytometry .RESULTS:Compared with normal control group , the cell proliferation activity in UVB group de-creased significantly ( P<0.05 ) .The cell activity was inversely correlated with the irradiation intensity .No significant difference of the cell activity between Na 2 SeO3 group and normal control group was observed .The cell proliferation in Na2SeO3 +UVB group was higher than that in UVB group significantly (P<0.05).Na2SeO3 at concentration of 100 nmol/L showed the strongest activity to promote cell proliferation .After 300 J/m2 UVB irradiation, the apoptotic rate in Na2SeO3+UVB group decreased significantly ( P<0.05) compared with UVB group .The inhibitory effect of Na 2 SeO3 at concentra-tion of 100 nmol/L on apoptosis was the strongest .CONCLUSION: The damage of human keratinocytes by UVB irradia-tion is in a dose-dependent manner .The photoprotection performance of Na 2 SeO3 reduces the damage of human keratino-cytes induced by UVB irradiation .