CAJ | 학술논문

目的：通过检测人胃癌组织及其癌旁组织中的RNA氧化损伤程度，探讨RNA氧化与胃癌发生的相关性。方法：分别利用免疫组化方法和液相-串联质谱（ LC-MS／MS）方法对61例胃癌组织及其癌旁组织中8-氧鸟苷（8-oxoGsn）进行定性和定量分析，并统计分析结果。结果：免疫组化结果检测显示8-oxoGsn在癌旁组织中含量低，而在胃癌组织中含量明显增高，且棕色染色区域主要集中在肿瘤细胞胞浆位置。质谱检测结果显示胃癌组织中8-oxoGsn含量较对应癌旁组织高，差异有统计学意义（ P＜0.05）。结论：8-oxoGsn在胃癌组织中含量增加。RNA氧化损伤可能在胃癌的发生发展过程中起重要作用。
목적：통과검측인위암조직급기암방조직중적RNA양화손상정도，탐토RNA양화여위암발생적상관성。방법：분별이용면역조화방법화액상-천련질보（ LC-MS／MS）방법대61례위암조직급기암방조직중8-양조감（8-oxoGsn）진행정성화정량분석，병통계분석결과。결과：면역조화결과검측현시8-oxoGsn재암방조직중함량저，이재위암조직중함량명현증고，차종색염색구역주요집중재종류세포포장위치。질보검측결과현시위암조직중8-oxoGsn함량교대응암방조직고，차이유통계학의의（ P＜0.05）。결론：8-oxoGsn재위암조직중함량증가。RNA양화손상가능재위암적발생발전과정중기중요작용。
AIM:To investigate the RNA oxidative damage in human gastric cancer tissue and para-carcinoma tissue for exploring the role of RNA oxidation in the occurrence of gastric cancer .METHODS:Immunohistochemical ob-servation and LC-MS/MS analysis were performed in 61 cases of gastric carcinoma .The position and concentration of 8-oxoguanosine ( 8-oxoGsn ) were detected , respectively . RESULTS: The results of immunohistochemical observation showed that 8-oxoGsn was obviously up-regulated in the gastric cancer .The positive staining mainly accumulated in the cy-toplasm of the tumor cells .The results of mass spectrometry showed that the level of 8-oxoGsn in the gastric cancer tissues was higher than that in the para-carcinoma tissues (P<0.05).CONCLUSION:8-oxoGsn is up-regulated in gastric canc-er.RNA oxidative damage may play important roles in the occurrence of gastric cancer .