中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
1期
154-159
,共6页
郎艳松%秘红英%刘美之%袁国强
郎豔鬆%祕紅英%劉美之%袁國彊
랑염송%비홍영%류미지%원국강
外膜%炎症%动脉粥样硬化%单核细胞趋化蛋白-1%白细胞介素-8%白细胞介素-10
外膜%炎癥%動脈粥樣硬化%單覈細胞趨化蛋白-1%白細胞介素-8%白細胞介素-10
외막%염증%동맥죽양경화%단핵세포추화단백-1%백세포개소-8%백세포개소-10
KEY WORDS] Adventitia%Inflammation%Atherosclerosis%Monocyte chemoattractant protein 1%Interleukin-8%Interleukin-10
目的:探讨通心络联合阿托伐他汀、阿司匹林对家兔动脉粥样硬化早期颈动脉外膜炎症因子表达的影响。方法:高脂饮食复合单侧颈总动脉硅胶管包裹制备兔动脉粥样硬化早期模型。72只新西兰兔随机分为对照组(control)、模型组(model)、通心络(tongxinluo)组、阿托伐他汀(atorvastatin)组、阿司匹林(aspirin)组和三药联用( three-drug combination )组。每组各12只。对照组给予普通饲料,模型组及各用药组家兔均实施单侧颈动脉硅胶管包裹术复合高脂饲料喂养,通心络组给予通心络超微粉混悬液(0.3 g· kg-1· d-1)灌胃,阿托伐他汀组给予阿托伐他汀(2.5 mg· kg-1· d-1)灌胃,阿司匹林组给予阿司匹林(12 mg· kg-1· d-1)灌胃,三药联用组给予通心络超微粉混悬液(0.3 g· kg-1· d-1)、阿托伐他汀(2.5 mg· kg-1· d-1)和阿司匹林(12.5 mg· kg -1· d-1)灌胃,连续给药,4周后取材。 HE染色判定颈动脉内中膜病理形态变化;生化法检测血脂变化;ELISA法测定各组家兔颈动脉包裹段外膜单核细胞趋化蛋白-1(MCP-1)、白细胞介素-1β(IL-1β)和IL-10表达水平;免疫组化法检测血管外膜IL-8表达水平。结果:模型组与对照组比较,血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)的水平明显增高。除阿司匹林组外,各用药组TC、TG和LDL-C的水平与模型组比较明显降低。三药联用组较其它用药组TC、TG和LDL-C的水平明显降低。模型组颈动脉外膜中MCP-1和IL-1β的表达明显高于对照组,IL-10的表达减弱。与模型组比较,各用药组颈动脉外膜中MCP-1和IL-1β的表达减弱,IL-10的表达增强。三药联用组较其它各用药组MCP-1和IL-1β的表达明显减弱,IL-10的表达明显增强。各用药组颈动脉外膜IL-8表达减少。结论:通心络、阿托伐他汀和阿司匹林三药联用可通过降脂、调节血管外膜炎症反应而有效延缓动脉粥样硬化进程,且较单药应用作用更佳。
目的:探討通心絡聯閤阿託伐他汀、阿司匹林對傢兔動脈粥樣硬化早期頸動脈外膜炎癥因子錶達的影響。方法:高脂飲食複閤單側頸總動脈硅膠管包裹製備兔動脈粥樣硬化早期模型。72隻新西蘭兔隨機分為對照組(control)、模型組(model)、通心絡(tongxinluo)組、阿託伐他汀(atorvastatin)組、阿司匹林(aspirin)組和三藥聯用( three-drug combination )組。每組各12隻。對照組給予普通飼料,模型組及各用藥組傢兔均實施單側頸動脈硅膠管包裹術複閤高脂飼料餵養,通心絡組給予通心絡超微粉混懸液(0.3 g· kg-1· d-1)灌胃,阿託伐他汀組給予阿託伐他汀(2.5 mg· kg-1· d-1)灌胃,阿司匹林組給予阿司匹林(12 mg· kg-1· d-1)灌胃,三藥聯用組給予通心絡超微粉混懸液(0.3 g· kg-1· d-1)、阿託伐他汀(2.5 mg· kg-1· d-1)和阿司匹林(12.5 mg· kg -1· d-1)灌胃,連續給藥,4週後取材。 HE染色判定頸動脈內中膜病理形態變化;生化法檢測血脂變化;ELISA法測定各組傢兔頸動脈包裹段外膜單覈細胞趨化蛋白-1(MCP-1)、白細胞介素-1β(IL-1β)和IL-10錶達水平;免疫組化法檢測血管外膜IL-8錶達水平。結果:模型組與對照組比較,血清總膽固醇(TC)、甘油三酯(TG)和低密度脂蛋白膽固醇(LDL-C)的水平明顯增高。除阿司匹林組外,各用藥組TC、TG和LDL-C的水平與模型組比較明顯降低。三藥聯用組較其它用藥組TC、TG和LDL-C的水平明顯降低。模型組頸動脈外膜中MCP-1和IL-1β的錶達明顯高于對照組,IL-10的錶達減弱。與模型組比較,各用藥組頸動脈外膜中MCP-1和IL-1β的錶達減弱,IL-10的錶達增彊。三藥聯用組較其它各用藥組MCP-1和IL-1β的錶達明顯減弱,IL-10的錶達明顯增彊。各用藥組頸動脈外膜IL-8錶達減少。結論:通心絡、阿託伐他汀和阿司匹林三藥聯用可通過降脂、調節血管外膜炎癥反應而有效延緩動脈粥樣硬化進程,且較單藥應用作用更佳。
목적:탐토통심락연합아탁벌타정、아사필림대가토동맥죽양경화조기경동맥외막염증인자표체적영향。방법:고지음식복합단측경총동맥규효관포과제비토동맥죽양경화조기모형。72지신서란토수궤분위대조조(control)、모형조(model)、통심락(tongxinluo)조、아탁벌타정(atorvastatin)조、아사필림(aspirin)조화삼약련용( three-drug combination )조。매조각12지。대조조급여보통사료,모형조급각용약조가토균실시단측경동맥규효관포과술복합고지사료위양,통심락조급여통심락초미분혼현액(0.3 g· kg-1· d-1)관위,아탁벌타정조급여아탁벌타정(2.5 mg· kg-1· d-1)관위,아사필림조급여아사필림(12 mg· kg-1· d-1)관위,삼약련용조급여통심락초미분혼현액(0.3 g· kg-1· d-1)、아탁벌타정(2.5 mg· kg-1· d-1)화아사필림(12.5 mg· kg -1· d-1)관위,련속급약,4주후취재。 HE염색판정경동맥내중막병리형태변화;생화법검측혈지변화;ELISA법측정각조가토경동맥포과단외막단핵세포추화단백-1(MCP-1)、백세포개소-1β(IL-1β)화IL-10표체수평;면역조화법검측혈관외막IL-8표체수평。결과:모형조여대조조비교,혈청총담고순(TC)、감유삼지(TG)화저밀도지단백담고순(LDL-C)적수평명현증고。제아사필림조외,각용약조TC、TG화LDL-C적수평여모형조비교명현강저。삼약련용조교기타용약조TC、TG화LDL-C적수평명현강저。모형조경동맥외막중MCP-1화IL-1β적표체명현고우대조조,IL-10적표체감약。여모형조비교,각용약조경동맥외막중MCP-1화IL-1β적표체감약,IL-10적표체증강。삼약련용조교기타각용약조MCP-1화IL-1β적표체명현감약,IL-10적표체명현증강。각용약조경동맥외막IL-8표체감소。결론:통심락、아탁벌타정화아사필림삼약련용가통과강지、조절혈관외막염증반응이유효연완동맥죽양경화진정,차교단약응용작용경가。
AIM:To investigate the effect of a treatment proposal , which consisted of tongxinluo , atorvastatin and aspirin, on adventitial inflammation of early atherosclerosis in rabbit carotid artery .METHODS: The atherosclerotic model was established in the rabbits with silicone collar , which was positioned around the carotid arterial adventitia +high-cholesterol diet .New Zealand rabbits ( n =72 ) were randomly divided into 6 groups ( n=12 ): control group , model group, tongxinluo group, atorvastatin group, aspirin group, and three-drug combination group.The rabbits in control group were fed with common foodstuffs , and the rabbits in all the other groups were fixed the right carotid arteries with the silicone tube, and were fed with fatty foodstuffs .The rabbits in tongxinluo group , atorvastatin group and aspirin group were given the suspension of tongxinluo supermicropowder (0.3 g· kg-1· d-1), atorvastatin (2.5 mg· kg-1· d-1) and aspirin (12 mg· kg-1· d-1) respectively,and the rabbits in three-drug combination group were given the suspension of tongxinluo su-permicropowder (0.3 g· kg-1· d-1), atorvastatin (2.5 mg· kg-1· d-1) and aspirin (12 mg· kg -1· d-1) together. The rabbits in each group were fed with the corresponding medicines for 4 weeks .The tissue slices of carotid artery were observed under light microscope with HE staining .The change of blood lipid was detected by biochemical assay .The pro-tein levels of MCP-1, IL-1βand IL-10 in the carotid arterial adventitia were detected by ELISA .The immunohistochemical staining was used to detect the protein expression of IL-8 around the carotid arterial adventitia .RESULTS:Compared with control group, the contents of TC, TG and LDL-C were significantly increased , and the content of IL-10 was significantly decreased in model group .The levels of TC , TG and LDL-C were significantly decreased in tongxinluo group and atorvasta-tin group compared with model group , no significant difference between tongxinluo group and atorvastatin group was ob -served.In the three-drug combination group, the levels of TC, TG and LDL-C were lower than those in atorvastatin group and tongxinluo group .Compared with control group , the contents of MCP-1 and IL-1βwere significantly increased , and the content of IL-10 was significantly decreased in model group .Compared with model group , the contents of MCP-1 and IL-1βwere decreased in tongxinluo group , atorvastatin group and aspirin group , no significant difference between the 3 groups was observed.The content of IL-10 was decreased in three-drug combination group , and the contents of TC , TG and LDL-C were lower than those in tongxinluo group , atorvastatin group and aspirin group .The content of IL-8 was decreased in tongxinluo group , atorvastatin group , aspirin group and three-drug combination group .CONCLUSION: The strategy of three-drug combination enhances the effect of regulating the lipid metabolism and inhibiting the adventitia inflammation .It plays an important role to intervene in the process of atherosclerosis .